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Distinct Cerebellar Glycosphingolipid Phenotypes in Wistar and Lewis Rats

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Abstract

Three strains of laboratory rats (Lewis, Wistar and Sprague–Dawley) commonly used for the study of movement disorders differ in gait traits. Sialic acid containing glycosphingolipids, called gangliosides, are abundant in the nervous system, where they affect numerous neurochemical events. Cerebellum plays the key role in the integration of body movements. This study investigated glycosphingolipid phenotypes with the hypothesis that they differ in cerebella of the three rat strains. In addition, forebrain and brain stem glycosphingolipid phenotypes were determined. Total glycosphingolipid fractions (neutral and acidic) were analysed by high performance thin-layer chromatography (HPTLC). Complex gangliosides were detected with HPTLC immunostaining by using cholera toxin B subunit after the neuraminidase pretreatment. The most significant differences were found in the cerebellum glycosphingolipid content. Lewis rats showed three fold higher monohexaosylceramides (galactosylceramide + sulfatide)/gangliosides ratio compared to Wistar rats. On the other hand, the cerebellum of Wistar rats contained an increased content of complex gangliosides GD1b, GT1b and GQ1b, fourfold, twofold and tenfold, respectively, compared to Lewis rats. This study shows that Wistar and Lewis rat strains have pronounced differences in glycosphingolipid content and the composition of cerebellum.

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ACKNOWLEDGMENTS

We express our warmest thanks to Dalibora Behmen for English language improvement.

Funding

This study was funded by Ministry of Science, Education and Sports Republic of Croatia, grant no. 216-2160133-0066.

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    Authors and Affiliations

    1. Clinical Department of Gynecology and Obstetrics, Split University Hospital Center, Split, Croatia

      Jasminka Rešić Karara

    2. Department of Medical Chemistry and Biochemistry, University of Split, School of Medicine, Šoltanska 2, 21000, Split, Croatia

      Martin Kowalski, Anita Markotić & Vedrana Čikeš Čulić

    3. Department of Biology, University of Split, School of Medicine, Split, Croatia

      Tatijana Zemunik

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    1. Jasminka Rešić Karara
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    2. Martin Kowalski
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    Correspondence to Vedrana Čikeš Čulić.

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    Ethical approval. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted, University of Split School of Medicine.

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    Jasminka Rešić Karara, Kowalski, M., Markotić, A. et al. Distinct Cerebellar Glycosphingolipid Phenotypes in Wistar and Lewis Rats. Neurochem. J. 14, 20–24 (2020). https://doi.org/10.1134/S1819712420010122

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    • DOI: https://doi.org/10.1134/S1819712420010122

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