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Lysozyme activity of the salivary gland secretion of the medicinal leeches H. verbana, H. medicinalis, and H. orientalis

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Abstract

Salivary gland secretions of three species of the medicinal leech differ in the level of their lysozyme, and peptidoglycan-lysing activity. Using a synthetic fluorogenic substrate, 4-methylumbelliferyltetra N-acetyl-β-chitotetraoxide, the glycosidase activity (as one of peptidoglycan-lysing activities) of salivary gland secretion of these species of the medicinal leech was quantitatively evaluated in comparison with egg lysozyme. It is suggested that lysozyme activity of the leech secretions is determined not only by 5 isoforms of destabilase-lysozyme, but by some other enzymes which can utilize these substrates including lysozymes other than i-type (invertebrate) lysozymes.

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References

  1. Baskova, I.P. and Isakhanyan, G.S., Girudoterapiya. Nauka i Praktika (Hirudotherapy. Science and Practice), Moscow: Monolit, 2004.

    Google Scholar 

  2. Blumental, N.L., Grekov Vestn. Khirurg., 1936, vol. 43, pp. 3–11.

    Google Scholar 

  3. Petrov, I.R., Lapkina, N.V., Kapitsa, L.M., and Protasov, N.N., Grekov Vestn. Khirurg., 1936, vol. 43, pp. 163–178.

    Google Scholar 

  4. Shpolyanskii, G.M., in Sbornik rabot po voenno-polevoi khirurgii i obshchei khirurgii, posvyashchennyi 30-letiyu nauchnoi deyatel’nosty A.V. Smirnova (Collection of Papers on Military Field Surgery and General Surgery, dedicated to XXXth anniversary of Scientific Activity of A.V. Smirnov), Leningrad, 1944, pp. 162–164.

  5. Baskova, I.P. and Zavalova, L.L., Bioorgan. Khim., 2008, vol. 34, pp. 337–343.

    CAS  Google Scholar 

  6. Baskova, I.P., Zavalova, L.L., Basanova, A.V., and Sass, A.V., Biochemistry (Moscow), 2001, vol. 66, pp. 1690–1697.

    Google Scholar 

  7. Zavalova, L., Baskova, I., Lukyanov, S., Sass, A., Snezhkov, E., Akopov, S., Artamonova, I., Archipova, V., Nesmeyanov, V., Kozlov, D., Benevolensky, S., Kiseleva, V., Poverenny, A., and Sverdlov, E., Biochim. Biophys. Acta, 2000, vol. 1478, pp. 69–77.

    Article  CAS  Google Scholar 

  8. Zavalova, L.L., Baskova, I.P., Barsova, E.V., Snezhkov, E.V., Akopov, S.B., and Lopatin, S.A., Biochemistry (Moscow), 2004, vol. 69, pp. 952–958.

    Article  Google Scholar 

  9. Miroshnikov, K.A., Chertkov, O.V., Nazarov, P.A., and Mesyanzhinov, V.V., Usp. Biol. Khim., 2006, vol. 46, pp. 65–98.

    CAS  Google Scholar 

  10. Yang, Y. and Hamaguchi, K., J. Biochem., 1980, vol. 87, pp. 1003–1014.

    CAS  Google Scholar 

  11. Yang, Y. and Hamaguchi, K., J. Biochem., 1980, vol. 88, pp. 829–836.

    CAS  Google Scholar 

  12. Bradford, M., Analyt. Biochem., 1976, vol. 72, pp. 248–254.

    Article  CAS  Google Scholar 

  13. Stone, K., Science, 2002, vol. 298, pp. 728–731.

    Article  CAS  Google Scholar 

  14. Baskova, I.P., Zavalova, L.L., Basanova, A.V., Moshkovskii, S.A., and Zgoda, V.G., Biochemistry (Moscow), 2004, vol. 69, pp. 945–951.

    Article  Google Scholar 

  15. Zavalova, L.L., Artamonova, I.I., Berezhnoy, S.N., Tagaev, A.A., Baskova, I.P., Andersen, J., Roepstorff, P. and Egorov, Ts.A., Biochem. Biophys. Res. Commun., 2003, vol. 306, pp. 318–323.

    Article  CAS  Google Scholar 

  16. Baskova, I.P., Kostryukova, E.S., Vlasova, M.A., Kharitonova, O.V., Levitskii, S.A., Zavalova, L.L., Moshkovskii, S.A., and Lazarev, V.N., Biochemistry (Moscow), 2008, vol. 73, pp. 388–394.

    Article  Google Scholar 

  17. Schulenburg, H. and Boehnisch, C., BMC Evolutionary Biology, 2008, vol. 8, pp. 114–133.

    Article  Google Scholar 

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Authors and Affiliations

  1. Biological Faculty, Lomonosov Moscow State University, Leninskye Gory 1/12, Moscow, 119991, Russia

    I. P. Baskova & L. L. Zavalova

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 119997, Russia

    O. V. Kharitonova

Authors
  1. I. P. Baskova
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  2. O. V. Kharitonova
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  3. L. L. Zavalova
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Correspondence to I. P. Baskova.

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Original Russian Text © I.P. Baskova, O.V. Kharitonova, L.L. Zavalova, 2012, published in Biomeditsinskaya Khimiya.

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Baskova, I.P., Kharitonova, O.V. & Zavalova, L.L. Lysozyme activity of the salivary gland secretion of the medicinal leeches H. verbana, H. medicinalis, and H. orientalis . Biochem. Moscow Suppl. Ser. B 6, 138–143 (2012). https://doi.org/10.1134/S1990750812020035

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  • DOI: https://doi.org/10.1134/S1990750812020035

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