概要
目的
考察S-腺苷甲硫氨酸(SAM)对淀粉酶产色链霉菌(Streptomyces diastatochromogenes)1628次级代谢产物产量的影响.
创新点
SAM是甲基的重要供体, 可为次级代谢产物提供前体, 同时也可以作为信号增强次级代谢产物合成基因的表达. 因此, 本研究首次探究了SAM与S. diastatochromogenes 1628次级代谢产物合成的相关性.
方法
以S. diastatochromogenes 1628为对象, 通过体外添加SAM以及在S. diastatochromogenes 1628体内过表达、 敲除和回补SAM编码基因metKsd, 考察S. diastatochromogenes 1628次级代谢产物产量的变化.
结论
由于渗透性差, 外源添加SAM对S. diastatochromogenes 1628合成次级代谢产物(丰加霉素和三种四烯大环内酯类抗生素: 四霉素A、 四霉素P和四烯菌素B)没有影响; 敲除、 回补和过表达基因metKsd证实增加胞内SAM可提高S. diastatochromogenes 1628三种四烯大环内酯类抗生素的产量, 且可促进四烯大环内酯类化合物合成关键基因的表达, 而胞内SAM浓度的变化对丰加霉素的产量和及其关键基因的表达均无明显影响.
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Acknowledgments
This study was supported by the Excellent Youth Fund of Zhejiang Province, China (No. LR17C140002).
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Yefeng HU, Juan WANG, and Jie XU conducted experiments. Yefeng HU wrote the original draft. Zheng MA designed the experiments and wrote this article. Andreas BECHTHOLD and Xiaoping YU gave some good suggestions on the revision of the manuscript, polished English and checked the final version. All authors have read and approved the final manuscript and, therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.
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Yefeng HU, Juan WANG, Jie XU, Zheng MA, Andreas BECHTHOLD, and Xiaoping YU declare that they have no conflict of interest.
This article does not involve any studies with human or animal subjects.
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Hu, Y., Wang, J., Xu, J. et al. Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628. J. Zhejiang Univ. Sci. B 22, 767–773 (2021). https://doi.org/10.1631/jzus.B2100115
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DOI: https://doi.org/10.1631/jzus.B2100115