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First Report of Molecular Characterization of Oochoristica hemidactyli Johri, 1955 (Cestoda, Linstowiidae) from the Common Wall Lizard, Hemidactylus brooki Gray, 1845 (Reptilia: Gekkonidae) Along with Morphological Redescription

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Abstract

Introduction

During an investigation, a rare cestode species belonging to the genus, Oochoristica were found in Meerut, UP, India, from the intestine of the host, Hemidactylus brookii Gray, 1845 (Reptilia: Gekkonidae). The tapeworms were found to be similar in their morphological characters with Oochoristica hemidactyli Johri, 1955 (Cestoda: Linstowiidae).

Materials and Methods

The host Hemidactylus brookii used in this analysis was caught from May to July 2018 from Department of Zoology, Chaudhary Charan Singh University, Meerut (UP), India. Parasites were examined by microscopy. 18S and ITS1-5.8S-ITS2 region of ribosomal RNA were sequenced and analyzed for the molecular study.

Results

This paper provides the first trustworthy confirmation about the occurrence of O. hemidactyli inferred with molecular data using ribosomal 18S and ITS1-5.8S-ITS2 gene cluster DNA sequences. Molecular analysis shows that the studied specimens were clustered separately and did not show a close relationship with any other cestode spp., represented in the GenBank.

Conclusion

This is the foremost molecular characterization of any species of Oochoristica and of O. hemidactyli after 64 years of its original description that contributing to the systematic analysis of the helminthological biodiversity of reptilian hosts. These results provide a reference for future molecular study of species of Oochoristica.

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Acknowledgements

We would like to acknowledge the Head, Department of Zoology, Chaudhary Charan Singh University, Meerut, for providing laboratory facilities.

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Authors and Affiliations

  1. Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut, UP, 250004, India

    Chandni Verma, Anshu Chaudhary, Bindu Sharma & Hridaya Shanker Singh

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  1. Chandni Verma
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  2. Anshu Chaudhary
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  4. Hridaya Shanker Singh
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Correspondence to Anshu Chaudhary.

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The authors declare that they have no conflict of interest.

Animal Rights Statement

All experiments and handling of host were according to the animal welfare guidelines and recommendations under Committee for the Purpose of Control And Supervision of Experiments on Animals (CPCSEA), India.

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Verma, C., Chaudhary, A., Sharma, B. et al. First Report of Molecular Characterization of Oochoristica hemidactyli Johri, 1955 (Cestoda, Linstowiidae) from the Common Wall Lizard, Hemidactylus brooki Gray, 1845 (Reptilia: Gekkonidae) Along with Morphological Redescription. Acta Parasit. 65, 250–255 (2020). https://doi.org/10.2478/s11686-019-00129-6

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