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Polyphasic characterization of the genus Anabaena from the Gulf of Gdańsk (Southern Baltic Sea). Application of DGGE in taxonomic studies on environmental samples

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Oceanological and Hydrobiological Studies

Abstract

The aim of this paper was a taxonomic verification of cyanobacteria of the genus Anabaena that occur in the Gulf of Gdańsk. Classical taxonomic methods were combined with modern molecular taxonomic methods (polyphasic approach). Analysis of the species diversity of cyanobacteria from the genus Anabaena was based on the microscopic analysis and on the analysis of 16S rRNA and ITS region. Comparison of the obtained results with sequences in GenBank showed 97.8–100% similarity for 16S rRNA and 98.8-100% similarity in the case of the ITS fragment. Similarity of the 16S rRNA and ITS sequence of 98.5% seems to be sufficient to determine the species.

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  1. Department of Marine Biology and Ecology Institute of Oceanography, University of Gdańsk, Al. Piłsudskiego 46, 81-378, Gdynia, Poland

    Grzegorz Siedlewicz, Karolina Forycka & Waldemar Surosz

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  1. Grzegorz Siedlewicz
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  2. Karolina Forycka
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  3. Waldemar Surosz
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Siedlewicz, G., Forycka, K. & Surosz, W. Polyphasic characterization of the genus Anabaena from the Gulf of Gdańsk (Southern Baltic Sea). Application of DGGE in taxonomic studies on environmental samples. Ocean and Hydro 41, 64–78 (2012). https://doi.org/10.2478/s13545-012-0029-8

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