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Identification of Genes for Resistance to Yellow Rust of Asian Origin in Winter Wheat Cultivars and Lines

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Abstract

Yellow rust caused by the fungus Puccinia striiformis West. (synonym P. glumarum Erikss. et Henn.) is a dangerous factor contributing to significantly lower yields of common wheat grain and its poor quality. Recent studies indicate that highly virulent strains of yellow rust expand their range from the near-Himalayan region and replace less pathogenic European races of P. striiformis, posing a real danger to the agricultural sector in Europe, including Ukraine, since most wheat varieties resistant to local races of P. striiformis are susceptible to these new highly virulent strains. In this regard, we assessed resistance to the yellow rust pathogen in 558 cultivars and lines of winter common wheat, in particular, 171 in the infection background and 387 in the natural background. Winter wheat samples in the collection variety trials originated from 17 countries, although most of them were of Ukrainian breeding. Screening of winter wheat cultivars and lines for yellow rust resistance showed that only 19 (or 3%) samples exhibited appropriate resistance. Analysis of the yellow rust-resistant wheat specimens using molecular markers for the Yr10 and Yr36 genes did not reveal samples containing either of these genes. At the same time, screening using molecular markers Xbarc8, S23M41-310, S23M41-140, and dp269 indicated the presence of DNA fragments of various lengths associated with resistance alleles of the genes Yr5, Yr15, and YrSp in four cultivars of Ukrainian breeding.

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Funding

This research was funded within the scientific and technical projects of the National Academy of Sciences of Ukraine “Development and Introduction of Molecular Genetic Methods for Detecting Genes for Resistance to Yellow Rust in Wheat” (0116U006101) and “Introduction of Molecular Genetic Markers of Resistance to Highly Virulent Yellow Rust Pathotypes of Asian Origin in the Wheat Breeding Process” (0117U002730).

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Authors and Affiliations

  1. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, 04123, Kyiv, Ukraine

    Ya. V. Pirko, A. V. Karelov, N. O. Kozub, B. V. Ivashchuk, T. V. Topchii & Ya. B. Blume

  2. Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine, 03022, Kyiv, Ukraine

    V. V. Morgun

  3. Institute of Plant Protection, National Academy of Agrarian Sciences of Ukraine, 03022, Kyiv, Ukraine

    A. V. Karelov, N. O. Kozub & I. A. Sozinov

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  1. Ya. V. Pirko
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  2. A. V. Karelov
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  3. N. O. Kozub
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  4. B. V. Ivashchuk
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  5. I. A. Sozinov
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  6. T. V. Topchii
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Correspondence to Ya. V. Pirko or Ya. B. Blume.

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The authors declare that they have no conflict of interests. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by K. Lazarev

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Pirko, Y.V., Karelov, A.V., Kozub, N.O. et al. Identification of Genes for Resistance to Yellow Rust of Asian Origin in Winter Wheat Cultivars and Lines. Cytol. Genet. 55, 227–235 (2021). https://doi.org/10.3103/S0095452721030075

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