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Effect of Implantation of a Fibrin Matrix Associated with Neonatal Brain Cells on the Course of an Experimental Spinal Cord Injury

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Abstract

The effect of immediate implantation of a fibrin matrix associated with allogeneic neonatal rat brain cells on their motor function recovery after a spinal cord injury (SCI) was studied. The cohorts of animals selected for the study were represented by white adult outbred rats (approximately 260 g each, 4 or 5 months old). The SCI simulation was based on a left-side hemisection of the spinal cord at the level of approximately T13–L1 segments. The rehabilitation treatment included immediate transplantation of a human fibrin matrix associated with neonatal rat brain cells (NBCs) (n = 9) into the injury area. The reference groups were represented by animals with isolated traumatic (Tr) SCIs (n = 7) and those with implantation of a human acellular fibrin (Fb) matrix (n = 6) into the injury area. The motor activity was assessed in the paretic hindlimb on the Basso, Beattie, and Bresnahan (BBB) scale; spasticity was evaluated on the Ashworth scale; and the pathohistological examination of longitudinal spinal cord sections sampled in the remote posttraumatic period was performed using the silver impregnation staining method. The fibrin matrix promotes viability, growth, and differentiation in the incorporated neonatal rat brain cells. Starting from the second or third week after the implantation into the injury epicenter, the motor function in the paretic limb corresponded to approximately 11 points in the Fb and NBC groups and to approximately six points in the Tr group on the BBB locomotor scale. No significant differences in the locomotor function of the paretic limb were recorded throughout the entire experiment between the NBC and Fb groups nor between the Fb and Tr groups. Significant differences between the NBC and Tr groups were recorded from the second, fourth, and eighth week, as well as the third and fifth months, post injury. A significant prevalence in the level of spasticity in the Tr group over the NBC and Fb groups was recorded, respectively, from the sixth and the seventh week after the injury. An immediate implantation of the fibrin matrix in complex with allogeneic neonatal brain cells or without the latter causes a significant positive effect on the motor function recovery after a lacerative SCI.

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This study was not supported by any grant from financial establishments of the governmental, commercial, or noncommercial sectors.

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Authors and Affiliations

  1. Bogomolets National Medical University, 01601, Kyiv, Ukraine

    V. V. Medvediev & S. I. Savosko

  2. Romodanov Institute of Neurosurgery, National Academy of Medical Sciences of Ukraine, 04050, Kyiv, Ukraine

    N. P. Oleksenko, L. D. Pichkur, S. A. Verbovska, N. G. Draguntsova & V. V. Vaslovych

  3. Kam’yanets-Podil’s’ka Municipal Hospital, 32301, Kam’yanets-Podil’s’kyi, Khmel’nyts’k oblast, Ukraine

    Yu. A. Lontkovskiy

  4. National Academy of Medical Sciences of Ukraine, 04050, Kyiv, Ukraine

    V. I. Tsymbalyuk

Authors
  1. V. V. Medvediev
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  2. N. P. Oleksenko
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  3. L. D. Pichkur
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  4. S. A. Verbovska
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  5. S. I. Savosko
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  6. N. G. Draguntsova
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  7. Yu. A. Lontkovskiy
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  8. V. V. Vaslovych
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  9. V. I. Tsymbalyuk
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Corresponding authors

Correspondence to V. V. Medvediev, N. P. Oleksenko, L. D. Pichkur, S. A. Verbovska, S. I. Savosko, N. G. Draguntsova, Yu. A. Lontkovskiy, V. V. Vaslovych or V. I. Tsymbalyuk.

Ethics declarations

Conflict of interests. The authors declare that they have no conflict of interests.

Statement on the welfare of animals. The study observed all standards of bioethics and humane treatment of animals, which were stipulated under the EU Council Directive 86/609/EEC On the Approximation of Laws, Regulations, and Administrative Provisions of the Member States regarding the Protection of Animals Used for Experimental and Other Scientific Purposes (1986), the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (1986), and Ukrainian Law no. 3447-IV On the Protection of Animals from Cruelty (2006). The investigations were sanctioned by the Commission on Bioethics of the Romodanov Neurosurgery Institute, National Academy of Medical Sciences of Ukraine (protocol no. 30 of April 11, 2019).

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Translated by N. Tarasyuk

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Medvediev, V.V., Oleksenko, N.P., Pichkur, L.D. et al. Effect of Implantation of a Fibrin Matrix Associated with Neonatal Brain Cells on the Course of an Experimental Spinal Cord Injury. Cytol. Genet. 56, 125–138 (2022). https://doi.org/10.3103/S0095452722020086

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  • DOI: https://doi.org/10.3103/S0095452722020086

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