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Effect of Proteinase from Aspergillus fumigatus on Blood Plasma Proteins

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Abstract

Extracellular proteinase of the opportunistic Aspergillus fumigatus D-1 strain (molecular weight ~33 kDa, pI 4.6) was isolated. It was shown that proteinase hydrolyzes casein, fibrin, fibrinogen, albumin, and hemoglobin to varying degrees. However, proteolytic activity with respect to globular proteins of blood plasma was comparable to fibrinolytic activity. Proteinase did not coagulate human fibrinogen and bovine fibrinogen; it also did not coagulate human and rabbit blood plasma without dilution and when diluted twice. The plasminogen-activating activity of A. fumigatus D-1 extracellular proteinase was found, which may indicate its ability to indirect fibrinolysis.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    A. A. Osmolovskiy, A. V. Orekhova, V. G. Kreyer & N. A. Baranova

  2. Faculty of Science I, Halle-Wittenberg Martin Luther University, 06108, Halle, Germany

    L. Schmidt

  3. Faculty of Pharmacology and Medicine, University of Rome “La Sapienza”, 00185, Rome, Italy

    A. V. Orekhova

  4. International Biotechnological Center, Moscow State University, 119234, Moscow, Russia

    N. S. Egorov

Authors
  1. A. A. Osmolovskiy
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  2. L. Schmidt
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  3. A. V. Orekhova
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  4. V. G. Kreyer
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  5. N. A. Baranova
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  6. N. S. Egorov
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Correspondence to A. A. Osmolovskiy.

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The authors declare that they have no conflict of interests. The studies were carried out without the use of animals and without the involvement of humans as subjects.

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Translated by K. Lazarev

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Osmolovskiy, A.A., Schmidt, L., Orekhova, A.V. et al. Effect of Proteinase from Aspergillus fumigatus on Blood Plasma Proteins. Moscow Univ. Biol.Sci. Bull. 76, 71–76 (2021). https://doi.org/10.3103/S0096392521020048

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  • DOI: https://doi.org/10.3103/S0096392521020048

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