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The structure of the intestinal microbiota of the intestine and the frequency of detection of pathogenicity genes (stx1, stx2, bfp) in Escherichia coli with normal enzymatic activity isolated from children during the first year of life

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Abstract

E. coli is an intestinal commensal of vertebrates. The ability to produce Shiga toxins (STX) is an important characteristic of the virulence feature of the Shiga toxin that is produced by E. coli (STEC). These potent cytotoxins block protein synthesis by inactivating ribosomes. Their action on the target cells is responsible for the most severe forms of STEC-induced disease, such as hemorrhagic colitis and the life-threatening hemolytic-uremic syndrome (HUS). Enteropathogenic E. coli (EPEC) also continues to be an important cause of infantile diarrhea in developing countries. Bundle-forming pili (bfp) are essential for the full virulence of enteropathogenic E. coli. Exchange of genetic material between different types of bacteria, as well as with other members of the family Enterobacteriaceae in the intestinal ecosystem, leads to the appearance of normal variants of E. coli with phenetic features of pathogenicity that can serve as a theoretical basis for attributing these strains to pathobionts. PCR was used to examine 68 strains of E. coli (E. coli with normal enzymatic activity) for the presence of genes encoding the synthesis of Shiga toxins (stx1 and stx2) and genes encoding the bundle-forming pilus (bfp). They were isolated from children with functional disorders of the gastrointestinal tract. The presence of the desired amplicon specific for stx1 and bfp genes has resulted in the formation of E. coli strains with more intense pathogenicity.

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References

  1. Khavkin, A.L. and Zhikhareva, N.S, Nowadays correction of dysbiotic changes in the children’s intestinal tract, Russ. Med. Zh. Det. Gastroenterol. Nutritsiol., 2004, vol. 12, no. 16, pp. 3–6.

    Google Scholar 

  2. Mambetova, E.F, Comparative characteristics of some biological properties of monocultures and coculture variations in bacteria of the genus Serratia and Staphylococcus aureus, Cand. Sci. (Med.) Dissertation, Chelyabinsk, 2007.

    Google Scholar 

  3. Akoev, Yu.S., Sentsova, T.B., and Yatsyn, G.V, Fresh view at the dysbiosis in newborns, Ross. Pediatr. Zh., 2000, no. 5, pp. 13–14.

    Google Scholar 

  4. Ouwehand, A. and Salminen, S, The role of intestinal microflora for the development of the immune system in early childhood, Eur. J. Nutr., 2002, vol. 1, pp. 32–37.

    Google Scholar 

  5. Mamontova, L.M., Savilov, E.D., Protod’yakonov, A.P., and Markova, Yu.A., Infektsionnaya “agressivnost” okruzhayuchshei sredy: kontseptsiya mikrobiologicheskogo monitoringa (Infectious “Aggressiveness” of Environment: Conception for Microbiological Monitoring), Novosibirsk: Nauka, 2000.

    Google Scholar 

  6. Pozdeev, O.K, Molecular and genetic foundations of Enterobacteria pathogenicity, Prakt. Med., 2010, vol. 2, no. 41, pp. 84–88.

    Google Scholar 

  7. Limanskaya, I.V., Biological and environmental aspect of the Escherichia persistence, Cand. Sci. (Med.) Dissertation, Volgograd, 2006.

    Google Scholar 

  8. Karaev, Ya.M., Protective and immunogenic properties of Escherichia anatoxin, Cand. Sci. (Vet.) Dissertation, Krasnodar, 2008.

    Google Scholar 

  9. Layman, E.F., Sharkova, V.A., Mazur, M.E., and Prosyannikova, M.N, The way for molecular and genetic characterizing pathogenicity factors E. coli isolated from different operative wounds, Mezhdunar. Zh. Eksp. Obraz., 2012, no. 5, pp. 84–85.

    Google Scholar 

  10. Kaftyreva, L.A., Dolgikh, T.I., Lazareva, L.I., Podkolzin, A.T., Voytovich, M.A., and Galileyskaya, S.B, Verotoxin as the main laboratory criterion for intestinal infection caused by Escherichia coli O157 complicated by haemolytic uraemic syndrome in infants in the Omsk region, Klin. Lab. Diagn., 2010, no. 8, pp. 52–53.

    Google Scholar 

  11. Scotland, S.M., Smith, H.R., Willshaw, G.A., and Rowe, B, Verocytotoxin production in strains of Escherichia coli is determined by genes carried on bacteriophage, Lancet, 1983, vol. 322, p. 216.

    Article  Google Scholar 

  12. Law, D, Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E. coli, J. Appl. Microbiol., 2000, vol. 88, pp. 729–745.

    Article  CAS  PubMed  Google Scholar 

  13. Ivanova, E.I., Popkova, S.M., Dzhioev, Yu.P., Rakova, E.B., Nemchenko, U.M., and Rychkova, L.V, The way for identifying Shiga toxin-producing Escherichia coli strains in populations of normal intestinal microbiota in infants with functional disorders of gastrointestinal tract, Klin. Lab. Diagn., 2014, vol. 59, no. 11, pp. 56–60.

    CAS  PubMed  Google Scholar 

  14. Ivanova, E.I, Formation of pathogenic potential in Escherichia coli auto-strains in colonic associative symbiosis in children with functional disorders of the gastrointestinal tract, Cand. Sci. (Biol.) Dissertation, Irkutsk, 2014.

    Google Scholar 

  15. Ivanova, E.I., Popkova, S.M., Dzhioev, Yu.P., and Rakova, E.B, Detection of pathogenicity genes encoding ability to toxin production in Escherichia coli strains isolated from children’s intestinal biotope, Byull. Vost.- Sib. Nauchn. Tsentra Sib. Otd. Ross. Akad. Med. Nauk, 2013, no. 2 (90), pp. 111–114.

    Google Scholar 

  16. Bukharin, O.V., Kremleva, E.A., and Sgibnev, A.V, The role of associative microsymbionts in the functioning of associative symbiosis, Zh. Mikrobiol., 2012, no. 6, pp. 89–95.

    Google Scholar 

  17. Gabrielyan, I.N., Gorskaya, E.M., Spirina, T.S., and Preobrazhenskaya, T.B, Enterococcus as pathogens of infectious post-operative complications, Zh. Mikrobiol., 2007, no. 4, pp. 50–53.

    Google Scholar 

  18. Popkova, S.M., Volokitina, A.S., Dzhioev, Yu.P., Medvedeva, P.A., Kozlova, L.S., Nemchenko, U.M., et al., Associations of Enterococcus species and pathogenicity genes isolated from different biotopes of Irkutsk’ inhabitants, Izv. Irkutsk. Gos. Univ. Ser. Biol. Ekol., 2011, vol. 4, no. 1, pp. 14–25.

    Google Scholar 

  19. Microbial Associations and Their Role in Infectious Process. http://www.medon-line.ru/microbiology/id/59.

  20. Onishchenko, G.G, Epidemiological situation in the Russian Federation and the main ways for its stabilization, Materialy dokladov 8-ogo Vserossiiskogo s”ezda epidemiologov, mikrobiologov i parazitologov (Proc. 8th All-Russian Convention of Epidemiologists, Microbiologists and Parasitologists), Moscow, 2002.

    Google Scholar 

  21. Bielaszewska, M., Mellmann, A., Zhang, W., Kock, R., Fruth, A., Bauwens, A., et al., Characterization of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: A microbiological study, Lancet Infect. Dis., 2011, vol. 11, pp. 671–676.

    CAS  PubMed  Google Scholar 

  22. Scheutz, F., Nielsen, E.M., Frimodt-Moller, J., Boisen, N., Morabito, S., Tozzoli, R., et al., Characteristics of the enteroaggregative Shiga toxin/verotoxin-producing Escherichia coli O104: H4 strain causing the outbreak of haemolytic uraemic syndrome in Germany, May to June 2011, Eurosurveillance, 2011, vol. 16, no. 24, p. 2. http://www.eurosurveillance.org/viewarticle. aspx?articleid=19889.

    Google Scholar 

  23. Egorova, S.A., Makarova, M.A., and Kaftyreva, L.A, The etiological significance of opportunistic enterobacteria in acute intestinal diseases and dysbiotic intestinal states, Infekts. Immun., 2011, vol. 2, no. 1, pp. 181–184.

    Google Scholar 

  24. Schmidt, H., Bielaszewska, M., and Karch, H, Transduction of enteric Escherichia coli isolates with a derivative of Shiga toxin 2-encoding bacteriophage phi3538 isolated from Escherichia coli O157: H7, Appl. Environ. Microbiol., 1999, vol. 65, pp. 3855–3861.

    CAS  PubMed  PubMed Central  Google Scholar 

  25. Tozzoli, R., Grande, L., Michelacci, V., Ranieri, P., Maugliani, A., Caprioli, A., et al., Shiga toxin-converting phages and the emergence of new pathogenic Escherichia coli: A world in motion, Front. Cell. Infect. Microbiol., 2014, vol. 4, no. 80. doi 10.3389/fcimb.2014.00080

  26. McDaniel, T.K. and Kaper, J.B., A cloned pathogenicity island from en-teropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K-12, Mol. Microbiol., 1997, vol. 23, pp. 399–407. doi 10.1046/j.1365-2958.1997.2311591.x

    Article  CAS  PubMed  Google Scholar 

  27. Levine, M.M, Escherichia coli that cause diarrhea: Enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent, J. Infect. Dis., 1987, vol. 155, pp. 377–389. doi 10.1093/infdis/155.3.377

    CAS  PubMed  Google Scholar 

  28. Ivanova, E.I., Popkova, S.M., Dzhioev, Yu.P., Rakova, E.B., Dolgikh, V.V., Savel’kaeva, M. V., et al., The way for determining degree of incidence of the genes encoding ability for forming pili binds in autostrains Escherichia coli, Klin. Lab. Diagn., 2015, vol. 60, no. 1, pp. 52–55.

    CAS  PubMed  Google Scholar 

  29. Avetisyan, L.R, Epidemiological and microbiological aspects on colonizing infants’ intestinal tract by opportunistic pathogens, Cand. Sci. (Med.) Dissertation, Moscow, 2008.

    Google Scholar 

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Authors and Affiliations

  1. Scientific Center of Family Health and Human Reproduction Problems, Irkutsk, 664003, Russia

    E. I. Ivanova, L. V. Rychkova, U. M. Nemchenko, E. V. Bukharova, M. V. Savelkaeva & Yu. P. Dzhioev

  2. Irkutsk State Medical University, Irkutsk, 664003, Russia

    Yu. P. Dzhioev

Authors
  1. E. I. Ivanova
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  2. L. V. Rychkova
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  3. U. M. Nemchenko
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  4. E. V. Bukharova
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  5. M. V. Savelkaeva
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  6. Yu. P. Dzhioev
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Correspondence to E. I. Ivanova.

Additional information

Original Russian Text © E.I. Ivanova, L.V. Rychkova, U.M. Nemchenko, E.V. Bukharova, M.V. Savelkaeva, Yu.P. Dzhioev, 2017, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2017, No. 1, pp. 36–40.

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Ivanova, E.I., Rychkova, L.V., Nemchenko, U.M. et al. The structure of the intestinal microbiota of the intestine and the frequency of detection of pathogenicity genes (stx1, stx2, bfp) in Escherichia coli with normal enzymatic activity isolated from children during the first year of life. Mol. Genet. Microbiol. Virol. 32, 42–48 (2017). https://doi.org/10.3103/S0891416817010062

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  • DOI: https://doi.org/10.3103/S0891416817010062

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