Abstract
Molecular genetic analysis of the rabies virus strain Moscow 3253 genome adapted to Vero cells. The isolated cDNA was specifically enriched with the nucleotide sequence of the target virus genome using amplification. Whole genome sequencing was performed on the Ion PGM platform and additionally using the AB 3500xl genetic analyzer. Alignment and assembly of sequences into a complete genome and further analysis were performed using DNASTAR Lasergene 11.2 and MEGA 7. Phylogenetic analysis was carried out using 479 rabies virus genomes represented in the NCBI GenBank database. The dendrogram was constructed using the BioNumerics 7.6 software package. The genome of the rabies virus Moscow 3253 (Vero) has been deposited into NCBI GenBank (accession MF630920). The genome of the rabies virus Moscow 3253 (Vero), as compared to its original variant, has 1069 single mutations. Its phylogenetic closeness to various vaccine variants of the rabies virus genome represented in the NBCI GenBank database, which are also adapted to the Vero or BHK cell culture, has been established. A strong influence of the Vero cell culture as a reproduction medium for the rabies virus Moscow 3253 (Vero) on the acquisition of a large number of single mutations by its genome has been revealed. As has been stated in a previously published work, the physicochemical and biological properties of rabies immunoglobulin (RIG) obtained by the technology using the rabies virus strain Moscow 3253 (Vero) meet the requirements of the regulatory documentation for RIG. Thus, the use of the rabies virus adapted to the Vero cells for the production of a rabies vaccine is promising.
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Translated by M. Novikova
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Krasnov Ya.M.—https://orcid.org/0000-0002-4909-2394; e-mail: rusrapi@microbe.ru
Alkhova Zh.V.—https://orcid.org/0000-0001-7681-5773
Generalov S.V.—https://orcid.org/0000-0003-1461-5383
Tuchkov I.V.—https://orcid.org/0000-0003-0712-8659
Naryshkina E.A.—https://orcid.org/0000-0002-9190-099X
Sharapova N.A.—https://orcid.org/0000-0002-5289-7783
Abramova E.G.—https://orcid.org/0000-0002-8798-1547
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Krasnov, Y.M., Alkhova, Z.V., Generalov, S.V. et al. Whole Genome Sequencing and Phylogenetic Analysis of the Rabies Virus Strain Moscow 3253 Adapted to a Vero Cell Line. Mol. Genet. Microbiol. Virol. 35, 237–242 (2020). https://doi.org/10.3103/S0891416820040060
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DOI: https://doi.org/10.3103/S0891416820040060