Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2229)
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Table of contents (16 protocols)
Keywords
About this book
This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experimental procedures, metabolic burden, computational techniques to predict such burden from models, and how DNA parts can be engineered in mammalian cells to sense, and respond to, and intracellular signals in general. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Synthetic Gene Circuits: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Editors and Affiliations
Bibliographic Information
Book Title: Synthetic Gene Circuits
Book Subtitle: Methods and Protocols
Editors: Filippo Menolascina
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1032-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-1031-2Published: 07 January 2021
Softcover ISBN: 978-1-0716-1034-3Published: 07 January 2022
eBook ISBN: 978-1-0716-1032-9Published: 06 January 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 353
Number of Illustrations: 7 b/w illustrations, 87 illustrations in colour
Topics: Human Genetics, Biotechnology, Biological Techniques