Overview
- Editors:
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Helen O. McCarthy
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School of Pharmacy, Queen's University, Belfast, Belfast, United Kingdom
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Jonathan A. Coulter
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School of Pharmacy, Queen's University, Belfast, Belfast, United Kingdom
- Addresses the challenges of accurate quantification of nitric oxide in either in vitro or in vivo models with practical, step-by-step protocols
- Includes readily reproducible techniques crafted by leading experts
- Provides detailed notes sections, documenting specific information on particularly challenging aspects of the methodologies
- Includes supplementary material: sn.pub/extras
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Table of contents (15 protocols)
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- David G. Hirst, Tracy Robson
Pages 1-13
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Detection and Quantification of Nitric Oxide
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- Kai Rothkamm, Susanne Burdak-Rothkamm
Pages 17-25
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- Enika Nagababu, Joseph M. Rifkind
Pages 27-37
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- Barbora Piknova, Alan N. Schechter
Pages 39-56
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- Xiaoxiang Zheng, Kezhou Liu, Yong Yang
Pages 73-80
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- Yong Chool Boo, Gyeong In Mun, Sarah L. Tressel, Hanjoong Jo
Pages 81-89
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- Marcela A. Segundo, LuÃs M. Magalhães, Joana P.N. Ribeiro, Marlene Lúcio, Salette Reis
Pages 91-104
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- David N. Silverman, Chingkuang Tu
Pages 105-114
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- Angela Elia, Pietro Mario Lugarà , Cinzia Di Franco, Vincenzo Spagnolo
Pages 115-133
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- Anatoly F. Vanin, Alexander A. Timoshin
Pages 135-149
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Nitric Oxide Generation
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Front Matter
Pages 151-151
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- Sean O. Hynes, Cillian McCabe, Timothy O’Brien
Pages 153-168
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- George Han, Adam J. Friedman, Joel M. Friedman
Pages 187-195
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- Beihui Liu, James Hewinson, Haibo Xu, Francisco Montero, Carmen R. Sunico, Federico Portillo et al.
Pages 197-223
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Back Matter
Pages 225-226
About this book
The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.