Abstract
For several years, it was generally believed that tissue culture cells derived from mammalian sources could not be mutagenized by exposure to known mutagens (Szybalski et al., 1964; Morrow, 1964). However, in 1968, independent studies by Chu and Mailing (1968a b) and Kao and Puck (1968) revealed that gene mutations are induced when Chinese hamster cells are incubated for short periods in the presence of alkylating agents. Additional studies proved that the spontaneous mutation rate for mammalian tissue culture cells is actually several orders of magnitude lower than that assumed in the 1964 reports. Based on this knowledge, it became easy to understand how Szybalski and Morrow failed to demonstrate an enhancement of mutant frequency upon treatment of cells with mutagens; i.e., the background mutant frequency was too high to observe an increase arising from the induction of new mutants.
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Clive, D., Flamm, W.G., Patterson, J.B. (1973). Specific-Locus Mutational Assay Systems for Mouse Lymphoma Cells. In: Hollaender, A. (eds) Chemical Mutagens. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8972-3_4
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DOI: https://doi.org/10.1007/978-1-4615-8972-3_4
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