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Molecular Mechanism of Platelet Adhesion

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Mechanisms of Stimulus—Response Coupling in Platelets

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 192))

Abstract

This paper concerns the adhesion of platelets to subendothelial structures, as measured in perfusion systems such as that developed by Baumgartner and his collaborators (1,2) and subsequently modified by Sakariassen et al (3,4). It has been shown that platelet adhesion to subendothelium is defective, particularly at high shear rates, in two hereditary bleeding disorders: Bernard-Soulier syndrome, in which the platelets lack the membrane glycoprotein Ib, and von Willebrand’s disease, in which the high-molecular-weight multimeric component of the factor VIII complex is either deficient from the plasma or structurally and functionally defective (5,6). I shall attempt here to review the growing body of evidence relating to the interactions between vessel wall components, the factor VIII/von Willebrand factor (vWF) and the platelet membrane, which are evidently necessary for adhesion to occur. I shall not have time for a detailed consideration of the relative importance in this series of reactions of the various components of the subendothelium, which remains a subject of much controversy, but shall confine myself to a discussion of the active sites involved on the platelets themselves and the vWF.

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References

  1. H R Baumgartner and R Muggii, Adhesion and aggregation: Morphological demonstration and quantitation in vivo and in vitro, in: Platelets in Biology and Pathology. J L Gordon ed, Elsevier, Amsterdam (1976).

    Google Scholar 

  2. V T Turitto and H R Baumgartner, Platelet interaction with subendothelium in flowing rabbit blood: effect of blood shear rate, Microvase Res 17:58 (1979).

    Article  Google Scholar 

  3. K S Sakariassen, P A Bolhuis and J J Sixma, Platelet adherence to subendothelium of human arteries in pulsatile and steady flow, Thromb Res 19:547 (1980).

    Article  CAS  Google Scholar 

  4. KS Sakariassen, P A M M Aarts, P G de Groot, W P M Houdijk and J J Sixma, A perfusion chamber developed to investigate platelet interaction in flowing blood with human vessel wall cells, their extracellular matrix and purified components, J Lab Clin Med 102:522 (1983).

    CAS  Google Scholar 

  5. T B Tschopp, H J Weiss, H R Baumgartner, Decreased adhesion of platelets to subendothelium in von Willebrand’s disease. J Lab Clin Med 85:296 (1974).

    Google Scholar 

  6. H J Weiss, V T Turitto and H R Baumgartner, Effect of shear rate on platelet interaction with subendothelium in citrated and native blood. I.Shear-dependent decrease of adhesion in von Willebrand’s disease and the Bernard-Soulier syndrome, J Lab Clin Med 92:750 (1978).

    CAS  Google Scholar 

  7. K S Sakariassen, P A Bolhuis and J J Sixma, Human blood platelet adherence to artery subendothelium is mediated by factor VIII-von Willebrand factor bound to the subendothelium, Nature 279:636 (1979).

    Article  Google Scholar 

  8. P A Bolhuis, K S Sakariassen, H J Sander, B N Bouma and J J Sixma, Binding of factor VIII-von Willebrand factor to human arterial subendothelium precedes increased platelet adherence and enhances platelet spreading, J Lab Clin Med 97:568 (1981).

    CAS  Google Scholar 

  9. E A Jaffe, L W Hoyer and R L Nachman, Synthesis of von Willebrand factor by cultured human endothelial cells, Proc Nat Acad Sci USA 71:1906 (1974).

    Article  CAS  Google Scholar 

  10. J J Rand, I I Sussman, R E Gordon, S V Chu and V Solomon, Localization of factor VHI-related antigen in human vascular subendothelium, Blood 55:752. (1980).

    CAS  Google Scholar 

  11. M A Howard, D C Montomery and R M Hardisty, Factor VIII related antigen in platelets, Thromb Res 4:6l7 (1974)

    Article  Google Scholar 

  12. J Koutts, P N Walsh, E F Plow, B N Bouma and T S Zimmerman Active release of human platelet factor VIII-related antigen by adenosine diphosphate, collagen and thrombin, J Clin Invest 62:1255 (1978).

    Article  CAS  Google Scholar 

  13. H V Stel, K S Sakariassen, P G de Groot, J A van Mourik and J J Sixma, The von Willebrand factor in the vessel wall mediates platelet adherence, Blood In press (1984).

    Google Scholar 

  14. J A Katzmann, D K Mujwid, R S Miller and D N Fass, Monoclonal antibodies to von Willebrand’s factor: reactivity with porcine and human antigens, Blood 58:530 (1981).

    CAS  Google Scholar 

  15. B Sola, P Avner, Y Sultan, C Jeanneau and P Maisonneuve, Monoclonal antibodies against human factor VIII molecule neutralize antihemophilic factor and ristocetin cofactor activities, Proc Nat Acad Sci USA 79:183 (1982).

    Article  CAS  Google Scholar 

  16. H V Stel, K S Sakariassen, B J Scholte, E C I Veerman, Th H van der Kwast, Ph G de Groot, J J Sixma and J A van Mourik, Characterization of 25 monoclonal antibodies to factor VIII-von Willebrand factor: relationship between ristocetin-induced platelet aggregation and platelet adherence to subendo-thelium, Blood 63:l408 (1984).

    Google Scholar 

  17. D Meyer, T S Zimmerman, B Obert and T S Edgington, Hybridoma antibodies to human von Willebrand factor. I. Characterisation of seven clones, Brit J Haemat 57:597 (1984).

    CAS  Google Scholar 

  18. D Meyer, H R Baumgartner and T S Edgington, Hybridoma antibodies to human von Willebrand factor. II. Relative role of intramolecular loci in mediation of platelet adhesion to the sub-endothelium, Brit J Haemat 57:609 (1984).

    CAS  Google Scholar 

  19. J J Sixma, K S Sakariassen, H V Stel, W P M Houdijk, D W In der Maur, R J Hamer, P G de Groot and J A van Mourik, Functional domains on von Willebrand factor: recognition of discrete tryptic fragments by monoclonal antibodies inhibiting interaction of von Willebrand factor with platelets and with collagen, In press (1984).

    Google Scholar 

  20. M H M Doucet-de Bruine, J J Sixma, J Over and N H Beeser-Visser, Heterogeneity of human factor VIII. II Characterization of forms of factor VIII binding to platelets in the presence of ristocetin. J Lab Clin Med 92:96 (1978).

    CAS  Google Scholar 

  21. H R Galnick, S B Williams and D K Morisato, Efect of the multimeric structure of the factor VIII/von Willebrand factor protein on binding to platelets, Blood 58:387 (1981).

    Google Scholar 

  22. C M Kessler, C M Floyd, M E Rick, D M Krizek, S L Lee and H R Gralnick, Collagen-Factor VIII/von Willebrand factor protein interaction, Blood 63:1291 (1984).

    CAS  Google Scholar 

  23. J J Sixma, K S Sakariassen, N H Beeser-Visser, M Ottenhof-Rovers and P A Bolhuis, Adhesion of platelets to human artery subendo thelium: Effect of factor VIII-von Willebrand factor of various multimeric composition, Blood 63:128 (1984).

    CAS  Google Scholar 

  24. Z M Ruggeri and T S Zimmerman, Variant von Willebrand’s disease. Characterization of two subtypes by analysis of multimeric composition of factor VIII/von Willebrand factor in plasma and platelets, J Clin Invest 65:1318 (1980).

    Article  CAS  Google Scholar 

  25. K S Sakariassen, M Cattaneo, A vd Berg, Z M Ruggeri, P M Mannucci and J J Sixma, DDAVP enhances platelet adherence and platelet aggregate growth on human artery subendothelium, Blood 64:229 (1984).

    CAS  Google Scholar 

  26. C Prowse. Personal communication. (1984).

    Google Scholar 

  27. E P Kirby and D C B Mills, The interaction of bovine factor VIII with human platelets, J Clin Invest 56:491 (1975).

    Article  CAS  Google Scholar 

  28. L De Marco and S S Shapiro, Properties of a human asialo-factor VIII, aristocetin-independent platelet aggregating agent, J Clin Invest 68:321 (1981).

    Article  Google Scholar 

  29. K-J Kao, S V Pizzo and P A McKee, Demonstration and characterization of specific binding sites for factor VIII/von Willebrand factor on human platelets, J Clin Invest 63:650 (1979).

    Article  Google Scholar 

  30. M A Howard, R A Hutton and R M Hardisty, Hereditary giant platelet syndrome: a disorder of a new aspect of platelet function, Brit Med J ii:586 (1973).

    Article  Google Scholar 

  31. J P Caen, A T Nurden, C Jeanneau et al, Bernard-Soulier syndrome — a new platelet glycoprotein abnormality. Its relationship wtih platelet adhesion to subendothelium and with the factor VIII von Willebrand protein, J Lab Clin Med 87:586 (1976).

    CAS  Google Scholar 

  32. M B Zucker, S-J Kim, J McPherson and R A Grant, Binding of factor VIII to platelets in the presence of ristocetin, Brit J Haemat 35:535 (1977).

    Article  CAS  Google Scholar 

  33. G Tobelem, S Levy-Toledano, R Bredoux O et al, New approach to determination of specific functions of platelet membrane sites, Nature 203:427 (1976).

    Article  Google Scholar 

  34. C Ruan, G Tobelem, A J McMichael O et al, Monoclonal antibody to human platelet glycoprotein I. II. Effects on human platelet function, Brit J Haemat 49:511 (1981).

    Article  CAS  Google Scholar 

  35. H J Weiss, T B Tschopp, H R Baumgartner O et al, Decreased adhesion of giant (Bernard-Soulier) platelets to subendothelium. Further implications on the role of the von Willebrand factor in hemostasis, Amer J Med 57:920 (1974).

    Article  CAS  Google Scholar 

  36. T Fujimoto and J Hawiger, Adenosine diphosphate induces binding of von Willebrand factor to human platelets, Nature 297:154 (1982).

    Article  CAS  Google Scholar 

  37. T Fujimoto, S Ohara and J Hawiger, Thrombin-induced exposure and prostacyclin inhibition of the receptor for factor VIII/von Willebrand factor on human platelets, J Clin Invest 69:1212 (1982).

    Article  CAS  Google Scholar 

  38. G Di Minno, S S Shapiro, P M Catalano, L De Marco and S Murphy, The role of ADP secretion and thromboxane synthesis in factor VIII binding to platelets, Blood 62:l86.

    Google Scholar 

  39. Z M Ruggeri, R Bader and L De Marco, Glanzmann thrombasthenia: Deficient binding of von Willebrand factor to thrombin-stimulated platelets, Proc Natl Acad Sci USA 79:6038 (1982).

    Article  CAS  Google Scholar 

  40. Z M Buggeri, L De Marco, L Gatti, R Bader and R R Montgomery, Platelets have more than one binding site for von Willebrand factor, J Clin Invest 72:1 (1983).

    Article  Google Scholar 

  41. T J C Nokes, N A Mahmoud, G F Savidge, A H Goodall, D Meyer, T S Edgington and R M Hardisty, Von Willebrand factor has more than one binding site for platelets, Thromb Res 34:36l (1984).

    Google Scholar 

  42. D Meyer. Personal communication. (1984).

    Google Scholar 

  43. G Pietu, G Cherel, G Marguerie and D Meyer, Inhibition of von Willebrand factor-platelet interaction by fibrinogen, Nature 308:648 (1984).

    Article  CAS  Google Scholar 

  44. J Schullek, J Jordan and RR Montgomery, Interaction of von Willebrand factor with human platelets in the plasma milieu, J Clin Invest 73:421 (1984).

    Article  CAS  Google Scholar 

  45. T B Tschopp, H J Weiss and H R Baumgartner, Interaction of thrombasthenic platelets with subendothelium: Normal adhesion, absent aggregation, Experientia 31:113 (1975).

    Article  CAS  Google Scholar 

  46. H J Weiss, V T Turitto and H R Baumgartner. In preparation, (1984).

    Google Scholar 

  47. D Jones, J Fritschy, J Garson, T J C Nokes, J T Kemshead and R M Hardisty, A monoclonal antibody binding to human medullo-blastoma cells and to the platelet glycoprotein IIb-IIIa complex, Brit J Haemat 57:621 (1984).

    CAS  Google Scholar 

  48. K S Sakariassen, M Ottenhof-Rovers and J J Sixma, Factor VIII-von Willebrand factor requires calcium for facilitation of platelet adherence, Blood 63:996 (1984).

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Haematology and Oncology, Institute of Child Health and Hospital for Sick Children, Great Ormond Street, London, WC1N 3JH, UK

    R. M. Hardisty

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  1. R. M. Hardisty
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Editor information

Editors and Affiliations

  1. Department of Pharmacology, Royal College of Surgeons, London, England

    J. Westwick

  2. Thrombosis Research Unit, King’s College School of Medicine and Dentistry, London, England

    M. F. Scully  & V. V. Kakkar  & 

  3. Department of Pharmacology, University of Glasgow, Glasgow, Scotland

    D. E. MacIntyre

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© 1985 Plenum Press, New York

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Hardisty, R.M. (1985). Molecular Mechanism of Platelet Adhesion. In: Westwick, J., Scully, M.F., MacIntyre, D.E., Kakkar, V.V. (eds) Mechanisms of Stimulus—Response Coupling in Platelets. Advances in Experimental Medicine and Biology, vol 192. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-9442-0_29

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  • DOI: https://doi.org/10.1007/978-1-4615-9442-0_29

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-9444-4

  • Online ISBN: 978-1-4615-9442-0

  • eBook Packages: Springer Book Archive

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