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Methods of Prevention, Control and Elimination of Mycoplasma Infection

  • Chapter
Mycoplasma Infection of Cell Cultures

Part of the book series: Cellular Senescence and Somatic Cell Genetics ((CSSCG,volume 3))

Abstract

Mycoplasme infected cell cultures should not be used for experimental or diagnostic work. Many reports have been published in this text and elsewhere on the effects of mycoplasme infection on cultured cells. These reports represent only a small portion of the total effects of infection. In a particular infected culture, the possible simultaneous effects are numerous, including: alterations in nucleic and amino acid metabolism, depletion of nutrients from the medium, chromosomal damage, phenotypic alteration, enzyme induction, induction of interferon, and membrane changes. An alteration in one characteristic can induce additional changes in other characteristics. Aula and Nichols demonstrated that chromosome damage by mycoplasmes was caused by depletion of arginine from the medium (1). Therefore, the overall effect of infection can be the additive or synergistic total of all mycoplasmal effects. Some effects may not become immediately produced and may become apparent only after 10, 20 or more population doublings following infection. For these reasons, studies showing no apparent difference between infected and non-infected cultures as a rationale to use infected cultures should be viewed skeptically.

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References

  1. Aula, P. and W.W. Nichols. 1967. The cytogenetic effects of mycoplasma in human leucocyte cultures. J. Cell Physiol. 70: 281–289.

    Article  PubMed  CAS  Google Scholar 

  2. Barile, M.F., H,E. Hopps, M.W. Grabowski, D.B. Riggs and R. delGiudice. 1973. The identification and sources of mycoplasmas isolated from contaminated cell cultures. Ann. N.Y. Acad. Sci. 225: 251–54.

    Article  Google Scholar 

  3. McGarrity, G.J. 1977. Factors influencing microbiological detection of mycoplasmas in cell cultures. Submitted for publication.

    Google Scholar 

  4. McGarrity, G.J. 1976. Spread and control of mycoplasmal infection of cell cultures. In Vitro 12: 643–648.

    Article  PubMed  CAS  Google Scholar 

  5. McKeehan, W.L., K.A. McKeehan, S.L. Hamilton and R.G. Ham. 1977. Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein. In Vitro 13: 399–416.

    Article  PubMed  CAS  Google Scholar 

  6. Boone, C.W., N. Mantel, T.D. Caruso, Jr., E. Kazam and R.E. Stevenson. 1972. Quality control studies on fetal bovine serum used in tissue culture. In Vitro 7: 174–189.

    Google Scholar 

  7. Barile, M.F. and J. Kern. 1971. Isolation of Mycoplasma arginini from commercial bovine sera and its implication in contaminated cell cultures. Proc. Soc. Exp. Biol. Med. 138: 432–437.

    PubMed  CAS  Google Scholar 

  8. Bahn, A.K. 1972. Basic Medical Statistics pp. 182–183, Grune and Stratton, New York.

    Google Scholar 

  9. Gerwe, E.G. 1956. Safety testing of poliomyelitis vaccine by tissue culture technic. Dagli Atti del 2 Congresso Internazionale de Standardizazione Immunomicrobiologica. Rome.

    Google Scholar 

  10. Knudsen, L.F. 1949. Sample size of parenteral solutions for sterility testing. J. Amer. Pharm. Assoc. 38: 332–337.

    Article  CAS  Google Scholar 

  11. Brewer, J.H. 1957. In: Antiseptics, Disinfectants, Fungicides and Sterilization. Second edition (G.L. Reddish, ed.) Lea and Febiger. Philadelphia, Pa. pp. 160–161.

    Google Scholar 

  12. The United States Pharmocopeia, Nineteenth edition. 1975 Mack Printing Co., Easton, Pa.

    Google Scholar 

  13. Molander, C.W., A.J. Kniazeff, C.W. Boone, A. Paley and D.T. Imagawa. 1972. Isolation and characterization of viruses from fetal calf serum. In Vitro 7: 168–173.

    Article  Google Scholar 

  14. Federoff, S., V.J. Evans, H.E. Hopps, K.K. Sanford and C.W. Boone. 1972. Summary of proceedings of a workshop on serum for tissue culture purposes. In Vitro 7: 161–165.

    Google Scholar 

  15. Molander, C.W., A. Paley, C.W. Boone, A.J. Kniazeff and D.T. Imagawa. 1968. Studies on virus isolation from fetal calf serum. In Vitro 4: 148.

    Google Scholar 

  16. Barile, M.F., H.E. Hopps and M.W. Grabowski. 1977. Incidence and sources of mycoplasma contamination: a brief review. These proceedings.

    Google Scholar 

  17. Barile, M.F. 1973. Mycoplasma contamination of cell cultures: mycoplasma-virus-cell culture interactions. In: Contamination of Tissue Cultures ( J. Fogh, ed.) Academic Press, N.Y. pp. 517–520.

    Google Scholar 

  18. Gois, M., F. Sisak, F. Kuksa and M. Sovadina. 1975. Incidence and evaluation of the microbial flora in the lungs of pigs with enzootic pneumonia. Zbl. Ved. Med. B. 22: 205–219.

    Article  CAS  Google Scholar 

  19. Kaklamanis, E., L. Thomas, K. Stavropoulos, I. Borman and C. Boshwitz. 1969. Mycoplasmacidal action of normal tissue extracts. Nature 221: 860–862.

    Article  PubMed  CAS  Google Scholar 

  20. Barile, M.F. 1967. Mycoplasma and cell cultures. NCI Monograph 29: 201–204.

    Google Scholar 

  21. Murphy, W.H., C. Bullis, L. Dabick, R. Heyn and C.J. Zarafonetis. 1970. Isolation of mycoplasma from leukemic and nonleukemic patients. J. Nat. Cancer Inst. 45: 243–251.

    PubMed  CAS  Google Scholar 

  22. Barile, M.F., G.P. Bodey, J. Synder, D.B. Riggs and M.W. Grabowski. 1966. Isolation of Mycoplasma orale from leukemic bone marrow and blood by direct culture. J. Nat. Cancer Inst. 36: 155–159.

    Google Scholar 

  23. McGarrity, G.J. 1977. Effects of mycoplasmas on lymphocyte cell cultures. These proceedings.

    Google Scholar 

  24. Purcell, R.H., R.M. Chanock and D. Taylor-Robinson. 1969. Serology of the mycoplasmas of man. In: The Mycoplasmatales and the L-Phase of Bacteria ( L. Hayflick, ed.) Appleton-Century Crofts, New York pp. 221–264.

    Google Scholar 

  25. Kundsin, R.B. and J. Praznik. 1967. Pharyngeal carriage of mycoplasma species in healthy young adults. Amer. J. Epidemiol. 86: 579–593.

    CAS  Google Scholar 

  26. Kumagai, K., T. Iwabuch, Y. Hinuma, K. Yuri and N. Ishida. 1971. Incidence, species and significance of mycoplasma species in the mouth. J. Infect. Dis. 123: 16–21.

    Article  PubMed  CAS  Google Scholar 

  27. Razin, S., J. Michmann and Z. Shimshoni. 1964. The occurrence of mycoplasmas (pleuropneumonia-like organisms) (PPLO) in the oral cavity of dentulous and edentulous subjects. J. Dent. Res. 43: 402–405.

    Article  PubMed  CAS  Google Scholar 

  28. Crawford, Y.E. and W.H. Kraybill. 1967. The mixture of mycoplasmas of man. Ann. N.Y. Acad. Sci. 143: 411–421.

    Article  PubMed  CAS  Google Scholar 

  29. Halbert, M.M., J. Krober, D. Vesley and N. Vick. 1975. Catalog of pipetting aids and other safety devices for the biomedical laboratory. University of Minnesota School of Public Health, Minneapolis.

    Google Scholar 

  30. Dimmick, R.L., W.F. Vogl and M.A. Chatigny. 1973. Potential for accidental aerosol transmission in the biological laboratory. In: Biohazards in Biological Research ( A. Hellman, M.N. Oxman and R. Pollack, eds.). Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., pp. 246–266.

    Google Scholar 

  31. Kenny, M.T. and F.L. Sabel. 1968. Particle size distribution of Serratia marcescans aerosols created during common laboratory accidents. Appl. Microbiol. 10: 1146–1150.

    Google Scholar 

  32. Kundsin, R.B. 1968. Aerosols of mycoplasmas, L forms and bacteria: comparison of particle size, viability, and lethality of ultraviolet radiation. Appl. Microbiol. 16: 143–146.

    PubMed  CAS  Google Scholar 

  33. Wright, D.N., G.D. Bailey and M.T. Hatch. 1968. Survival of airborne mycoplasma as affected by relative humidity. J. Bacteriol. 95: 251–252.

    PubMed  CAS  Google Scholar 

  34. Gartler, S.N. 1967. Genetic markers as tracers in cell culture. NCI Monograph 26: 167–194.

    CAS  Google Scholar 

  35. Nelson-Rees, W.A. and R.R. Flandermeyer. 1977. Inter and intra species contamination of human breast tumor cell lines HBC and BrCa5 and other cell cultures. Science 195: 1343–1344.

    Article  PubMed  CAS  Google Scholar 

  36. McGarrity, G.J. 1975. Serum quality control. TCA Manual 1: 167–169.

    Article  Google Scholar 

  37. McGarrity, G.J. 1975. Control of microbiological contamination. TCA Manual 1: 181–184.

    Article  Google Scholar 

  38. McGarrity, G.J. and L.L. Coriell. 1971. Procedures to reduce contamination of cell cultures. In Vitro 6: 257–265.

    Article  PubMed  CAS  Google Scholar 

  39. Bruun, J., G.J. McGarrity, W.S. Blakemore and L.L. Coriell 1976. Epidemiology of Pseudomonas aeruginosa infection by pyocin typing. J. Clin. Microbiol. 3: 264–271.

    PubMed  CAS  Google Scholar 

  40. Armstrong, D. 1973. Contamination of tissue culture by bacteia and fungi. In: Contamination in Tissue Culture. ( J. Fogh, ed.). Academic Press, N.Y.

    Google Scholar 

  41. McGarrity, G.J. 1977. Cell culture facilities. TCA Manual. In press.

    Google Scholar 

  42. Hopps, H.E., B C. Meyer, M.F. Barile and R.A. DelGiudice. 1973. Problems concerning “non-cultivable” mycoplasma contaminants in tissue cultures. Ann. N.Y. Acad. Sci. 225: 265–276.

    Article  Google Scholar 

  43. DelGiudice, R.A. and H.E. Hopps. 1977. Microbiological methods and fluorescent microscopy for the direct demonstration of mycoplasmal infection of cell cultures. These proceedings.

    Google Scholar 

  44. McGarrity, G.J. and L.L. Coriell. 1973. Detection of anaerobic mycoplasmas in cell cultures. In Vitro 9: 17–18.

    Article  PubMed  CAS  Google Scholar 

  45. Anonymous. 1972. Federal Standard No. 209 b, U.S. Govt. Printing Office, Washington, D.C.

    Google Scholar 

  46. Whitby, K.T. and D.A. Lundgren. 1965. Mechanics of air cleaning. Trans. A.S.A.E. 8: 342–352.

    Google Scholar 

  47. Coriell, L.L. and G.J. McGarrity. 1968. Biohazard hood to prevent infection during microbiological procedures. Appl. Microbiol. 16: 1895–1900.

    PubMed  CAS  Google Scholar 

  48. National Sanitation Foundation Advisory Committee for biohazard Cabinetry. 1976. National Sanitation Foundation Standard No. 49 for Class I I ( Laminar Flow) Biohzard Cabinetry. NSF, Ann Arbor, Michigan.

    Google Scholar 

  49. Coriell, L.L., G.J. McGarrity and J. Horneff. 1967. Medical applications of dust-free rooms I: elimination of airborne bacteria in a research laboratory. J. Amer. J. Public Health Nat. Health 57: 1824–1836.

    Article  CAS  Google Scholar 

  50. Coriell, L.L., W.S. Blakemore and G.J. McGarrity. 1968. Medical applications of dust-free rooms: II. elimination of airborne bacteria from an operating theatre. J. Amer. Med. Ass. 203: 1038–1046.

    Article  CAS  Google Scholar 

  51. McGarrity, G.J., L.L. Coriell, A.E. Greene, R.W. Schaedler, and R.J. Mandle. 1969. Medical applications of dust-free rooms: use in an animal care laboratory. Appl. Microbiol. 18: 142–146.

    PubMed  CAS  Google Scholar 

  52. Wedum, A.G., W.E. Barkley and A. Hellman. 1972. Handling of infectious agents. J.Amer. Vet. Med. Ass. 16: 1557–1567.

    Google Scholar 

  53. Pollock, M.F. and G.E. Kenny. 1963. Mammalian cell cultures contaminated with pleuro-pneumonia-like organisms. III. elimination of pleuro-pneumonia like organisms with specific antiserum. Proc. Soc. Exp. Biol. Med. 112: 176–181.

    PubMed  CAS  Google Scholar 

  54. Perlman, D., S.B. Rahman and J.B. Seman. 1967. Antibiotic control of mycoplasma in cell culture. Appl. Microbiol. 15: 82–85.

    PubMed  CAS  Google Scholar 

  55. Gori, G.B. and D.Y. Lee. 1964. A method for eradication of mycoplasma infections in cell cultures. Proc. Soc. Exp. Biol. Med. 117: 918–921.

    PubMed  CAS  Google Scholar 

  56. Mardh, P.H. 1975. Elimination of mycoplasmas from cell cultures with sodium polyanethol sulphonate. Nature 254: 515–516.

    Article  PubMed  CAS  Google Scholar 

  57. VanDiggelen, O., S. Shin and D. Phillips. 1977. Reduction in cellular tumorigenicity after mycoplasma infection and elimination of mycoplasma from infected cultures by passage in nude mice. Cancer Res. 37: 2680–2687.

    CAS  Google Scholar 

  58. Stanbridge, E. 1971. Mycoplasmas and cell cultures. Bacteriol. Rev. 35: 206–227.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Microbiology, Institute for Medical Research, Camden, New Jersey, USA

    Gerard J. McGarrity, Veronica Vanaman & Judith Sarama

Authors
  1. Gerard J. McGarrity
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  2. Veronica Vanaman
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  3. Judith Sarama
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Editor information

Editors and Affiliations

  1. Institute for Medical Research, Camden, New Jersey, USA

    Gerard J. McGarrity  & Warren W. Nichols  & 

  2. National Institute on Aging, Bethesda, Maryland, USA

    Donald G. Murphy

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McGarrity, G.J., Vanaman, V., Sarama, J. (1978). Methods of Prevention, Control and Elimination of Mycoplasma Infection. In: McGarrity, G.J., Murphy, D.G., Nichols, W.W. (eds) Mycoplasma Infection of Cell Cultures. Cellular Senescence and Somatic Cell Genetics, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9874-5_15

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  • DOI: https://doi.org/10.1007/978-1-4684-9874-5_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-9876-9

  • Online ISBN: 978-1-4684-9874-5

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