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Metabolome Profiling of Growth Hormone Transgenic Coho Salmon by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

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Oceanography Challenges to Future Earth

Abstract

Growth in fish is regulated in part by the growth hormone (GH)-insulin-like growth factor (IGF) axis, and salmon transgenic for GH are known to show dramatic increased growth. However, little is known concerning the in vivo global levels of metabolites and the mechanism of enhancement of growth in GH transgenic vertebrates. The present study examined the charged metabolites levels in GH transgenic coho salmon (Oncorhynchus kisutch) overexpressing GH by metabolomic analysis. Triplicate groups of size-matched (0 year-old, approx. 60 g) and age-matched (1.5 years-old) GH transgenic (T) and non-transgenic (NT) wild salmon were quantitatively assessed for levels of approximately 200 metabolites in both muscle and liver. The most notable difference found between T and NT fish was that glycolysis metabolite levels were increased in the muscle of transgenic fish. In addition, an increase in some metabolite levels in the transgenic fish muscle was found to be enhanced by ration-restriction. However, these effects observed in muscle were different from that seen in liver. The results suggest that GH transgenesis can improve the use of carbohydrates as a source of energy associated with rapid growth. These effects are likely to depend on the level of total digestible energy intake and type of tissue in transgenic fish.

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Change history

  • 07 June 2019

    In the original version of the book, the following corrections have been incorporated:

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      Chapter 18 was published with incorrect figures and affiliations of the authors.

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Acknowledgements

The authors thank staff at Center for Aquaculture and Environment Research, Fisheries and Oceans Canada, Institute for Advanced Biosciences, Keio University, Japan, and Dr. T. Fujimoto at Hokkaido University, Japan for assistance with lab work. TN acknowledges Dr. J. G. Richards, Mr. D. Chung and Miss. H. Bryant at Department of Zoology, The University of British Columbia, Canada, and Drs. M. Sato, T. Yamaguchi, Y. Ochiai, and I. Gleadall at Tohoku University, Japan for valuable discussion. This study was supported in part by KAKENHI grant (#23580277) and a fund to Core-to-Core Program (A. Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety” from JSPS to TN and by the Canadian Regulatory System for Biotechnology to RHD.

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Authors and Affiliations

  1. Marine Biochemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, 980-8572, Japan

    Toshiki Nakano

  2. Nutrition Laboratory, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, 980-8572, Japan

    Hitoshi Shirakawa

  3. Institute of Metabolic Science, University of Cambridge, Addenbrooke’s Site B289, Addenbrookes Hosp Hills R, Robinson Way, Cambridge, CB2 0QQ, UK

    Giles Yeo

  4. Fisheries and Oceans Canada, 4160 Marine Drive, West Vancouver, BC,, V7V 1N6, Canada

    Robert H. Devlin

  5. Institute for Advanced Biosciences, Keio University, Mizukami, Tsuruoka, 997-0035, Japan

    Tomoyoshi Soga

Authors
  1. Toshiki Nakano
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  2. Hitoshi Shirakawa
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  3. Giles Yeo
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  4. Robert H. Devlin
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  5. Tomoyoshi Soga
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Corresponding author

Correspondence to Toshiki Nakano .

Editor information

Editors and Affiliations

  1. SFJO Japan, Masion franco-japonaise de Tokyo, Yokohama College of Commerce, Atmosphere and Ocean Research Institute, The University of Tokyo, Tokyo, Japan

    Teruhisa Komatsu

  2. SFJO France, Académie des Sciences, Lettres et Arts de Marseille, Marseille, France

    Hubert-Jean Ceccaldi

  3. SFJO Japan, Masion franco-japonaise de Tokyo, Department of Ocean Sciences, Laboratory of Physics and Environmental Modeling, Tokyo University of Marine Science and Technology, Tokyo, Japan

    Jiro Yoshida

  4. SFJO France, Paris, France

    Patrick Prouzet

  5. Politique maritime et gouvernance, Correspondant Asie-Pacifique (JAMSTEC), Institut Français pour l’Exploitation de la Mer (Ifremer), Issy-les-Moulineaux, France

    Yves Henocque

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Nakano, T., Shirakawa, H., Yeo, G., Devlin, R.H., Soga, T. (2019). Metabolome Profiling of Growth Hormone Transgenic Coho Salmon by Capillary Electrophoresis Time-of-Flight Mass Spectrometry. In: Komatsu, T., Ceccaldi, HJ., Yoshida, J., Prouzet, P., Henocque, Y. (eds) Oceanography Challenges to Future Earth. Springer, Cham. https://doi.org/10.1007/978-3-030-00138-4_18

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