Abstract
Biomarker testing has proved a very fertile field for innovation, the development and application of multiple alternative methods for identifying and assessing biomarker expression, in certain cases overlaid with detection and evaluation of any associated immune response. Different methods have differing advantages and disadvantages, including perceived accuracy, performance issues, cost and logistics. Many of these issues have been discussed in preceding chapters, and the choice of test method may simply be governed by what is possible, and affordable. Data relating comparing the pros and cons of the various methods have been well reviewed in a meta-analysis by Lu et al. (2019).
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References
Ahn S, Woo JW, Lee K, Park SY. HER2 status in breast cancer: changes in guidelines and complicating factors for interpretation. J Pathol Transl Med. 2020;54(1):34–44. https://doi.org/10.4132/jptm.2019.11.03.
Burlingame EA, McDonnell M, Schau GF, Thibault G, Lanciault C, Morgan T, Johnson BE, Corless C, Gray JW, Chang YH. SHIFT: speedy histological—to—immunofluorescent translation of whole slide images enabled by deep learning. bioRxiv. 2019. https://doi.org/10.1101/730309.; https://www.biorxiv.org/content/10.1101/730309v1.full.pdf
Chowdary MVP, Kumar KK, Thakur K, Anand J, Krishna CM, Mathew S. Discrimination of normal and malignant mucosal tissues of the colon by Raman spectroscopy. Photomed Laser Surg. 2007;25:269–74.
Foldesi B. Overview of multiplex immunohistochemistry. Biomol. 2020. https://www.biomol.com/resources/biomol-blog/overview-of-multiplex-immunohistochemistry
Gaifulina R, Lau K, Rodriquez-Justo M, Kendall CA. Light at the end of the tunnel: application of Raman spectroscopy in colon cancer diagnostics. Editorial. Austin J Clin Pathol. 2014;1:1021.
Giacomelli MG, Husvogt L, Vardeh H, Faulkner-Jones BE, Hornegger J, Connolly JL, Fujimoto JG. Virtual hematoxylin and eosin transillumination microscopy using epi-fluorescence imaging. PLoS One. 2016;11(8):e0159337. https://doi.org/10.1371/journal.pone.0159337.
Hauptmann G. In situ hybridization methods. New York, NY: Humana Press; 2015. https://doi.org/10.1007/978-1-4939-2303-8.
Heier S, Muralitharan S, Freeland J, von Bueren E, Gobetti A. Comparing IHC, IR and Raman spectroscopy for classification of tissue and cell lines containing different levels of HER2 expression. Abstract poster presentation. ThermoScientific. 2021. https://static.thermoscientific.com/images/D20334~.pdf. Accessed Feb 2021.
Huang Z, McWilliams A, Lam S, English J, McLean DI, Lui H, Zeng H. Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues. Int J Oncol. 2003;23(3):649–55.
Keren L, Bosse M, Thompson S, et al. MIBI-TOF: a multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv. 2019;5(10):eaax5851. https://doi.org/10.1126/sciadv.aax5851.
Kong K, Kendall C, Stone N, Notingher I. Raman spectroscopy for medical diagnostics—from in-vitro biofluid assays to in-vivo cancer detection. Adv Drug Deliv Rev. 2015;89:121–34.
Lee C-W, Ren YJ, Marella M, Wang M, Hartke J, Couto SS. Multiplex immunofluorescence staining and image analysis assay for diffuse large B cell lymphoma. J Immunol Methods. 2019;478:112714. https://doi.org/10.1016/j.jim.2019.112714.
Levenson RM, Borowsky AD, Angelo M. Immunohistochemistry and mass spectrometry for highly multiplexed cellular molecular imaging. Lab Investig. 2015;95:397–405. https://doi.org/10.1038/labinvest.2015.2.
Lewis AT, Gaifulina R, Guppy NJ, Isabelle M, Dorney J, Lloyd GR, Rodriguez-Justo M, Kendall C, Stone N, Thomas GM. Developing Raman spectroscopy as a diagnostic tool for label-free antigen detection. J Biophotonics. 2018;2:11. https://doi.org/10.1002/jbio.201700028.
Lin JR, Izar B, Wang S, Yapp C, Mei S, shah PM, Santagata S, Sorger PK. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. elife. 2018;7:e31657. https://doi.org/10.7554/eLife.31657.
Lu S, Stein JE, Rimm DL, et al. Comparison of biomarker modalities for predicting response to PD-1/PD-L1 checkpoint blockade a systematic review and meta-analysis. JAMA Oncol. 2019; https://doi.org/10.1001/jamaoncol.2019.1549.
Mukhopadhyay S, Feldman MD, Abels E, et al. Whole slide imaging versus microscopy for primary diagnosis in surgical pathology: a multicenter blinded randomized non-inferiority study of 1992 cases (pivotal study). Am J Surg Pathol. 2018;42:39–52. https://doi.org/10.1097/PAS.0000000000000948.
Orringer DA, Pandian B, Niknafs YS, et al. Rapid interoperative histology of unprocessed biopsy specimens via fibre-laser-based stimulated Raman scattering microscopy. Nat Biomed End. 2017;1:0027. https://doi.org/10.1038/s41551-106-0027.
Parra ER, Uraoka N, Jiang M, Cook P, Gibbons D, Forget M-A, Bernatchez C, Haymaker C, Wistuba II, Rodriguez-Canales J. Validation of multiplex immunofluorescence panels using multispectral microscopy for immune-profiling of formalin-fixed and paraffin-embedded human tumor tissues. Sci Rep. 2017;7:13380. https://doi.org/10.1038/s41598-017-13942-8.
Raman CV, Krishnan KS. A new type of secondary radiation. Nature. 1928;121:3048.
Taube JM, Akturk G, Angelo M, et al. The Society for Immunotherapy of cancer statement on the best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation. J ImmunoTherapy Cancer. 2020;8 https://doi.org/10.1136/jitc-2019-000155.
Taylor CR. Milestones in immunohistochemistry and molecular morphology. Appl Immunohistochem Mol Morph. 2020;28:83–94.
Taylor CR, Cote RJ, editors. Immunomicroscopy: a diagnostic tool for the surgical pathologist. 3rd ed. Saunders, PA: Elsevier; 2005. (1st edition, Taylor was 1986; 2nd edition, Taylor and Cote was 1994)
Tubbs RR, Wang H, Wang Z Minca EC, Portier BP, Am G, Lanigan C, Luo Y, Cook JR, Ma X-J. Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders. Am J Clin Pathol. 2013;140:736–46.
Tumeh PC, Harview CL, Yearly JH, Shinaku IP, EMJ T, et al. PD-1 blockade induces responses by inhibiting adaptive immune resistance. Nature. 2014;515:568–71. https://doi.org/10.1038/nature13954.
Valm A, Cohen S, Legant W, Melunis J, Hershberg U, Wait E, Cohen AR, Davidson MW, Betzig E, Lippincott-Schwartz J. Applying systems-level spectral imaging and analysis to reveal the organelle interactome. Nature. 2017;546:162–7. https://doi.org/10.1038/nature22369.
Wang F, Flanagan J, Su N, Wang L-C, Bui S, Nielson A, Wu X, Vo H-T, Ma X-J, Luo Y. RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn. 2012;14(1):22–9. https://doi.org/10.1016/j.jmoldx.2011.08.002.
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Jasani, B., Huss, R., Taylor, C.R. (2021). Immunofluorescence, In Situ Hybridization and Alternative Forms of ‘Labeled’ Microscopy. In: Precision Cancer Medicine. Springer, Cham. https://doi.org/10.1007/978-3-030-84087-7_14
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DOI: https://doi.org/10.1007/978-3-030-84087-7_14
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