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Large-Scale Proteome and Phosphoproteome Quantification by Using Dimethylation Isotope Labeling

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Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis

Part of the book series: Springer Theses ((Springer Theses))

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Abstract

Protein characterization alone is usually not enough to elucidate most biological processes. Although thousands of proteins can be identified in one proteomic experiment, it is difficult to relate these proteins with their biological functions. The expression levels of proteins within a living organism are reflections of the different physiological and pathological states. Conventional protein quantification technologies such as western blot (WB) are low throughput and can only quantify one protein in each experiment. Therefore, comprehensive proteome quantification in certain depth is an important direction in the development of proteomic technologies and is considered as the bridge for the gap between proteins and their biological function [1–7]. On the other hand, there are more than 300 types of posttranslational modifications (PTMs) that can dynamically modify the whole proteome, which makes the protein sample even more complex. The occupancy level of a PTM on specific site of a protein is also critical to the biological function of the protein in the regulation of different physiological and pathological processes, such as the protein phosphorylation is related to the signal transduction in many pathways activation and protein glycosylation is related to cell-to-cell recognition [8–10]. Therefore, comprehensive quantification of proteome PTMs is also another important task for current proteomic analysis.

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  1. Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, People’s Republic of China

    Fangjun Wang

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Correspondence to Fangjun Wang .

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Wang, F. (2014). Large-Scale Proteome and Phosphoproteome Quantification by Using Dimethylation Isotope Labeling. In: Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis. Springer Theses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-42008-5_4

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