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Bacterial Proteases and Virulence

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Regulated Proteolysis in Microorganisms

Part of the book series: Subcellular Biochemistry ((SCBI,volume 66))

Abstract

Bacterial pathogens rely on proteolysis for variety of purposes during the infection process. In the cytosol, the main proteolytic players are the conserved Clp and Lon proteases that directly contribute to virulence through the timely degradation of virulence regulators and indirectly by providing tolerance to adverse conditions such as those experienced in the host. In the membrane, HtrA performs similar functions whereas the extracellular proteases, in close contact with host components, pave the way for spreading infections by degrading host matrix components or interfering with host cell signalling to short-circuit host cell processes. Common to both intra- and extracellular proteases is the tight control of their proteolytic activities. In general, substrate recognition by the intracellular proteases is highly selective which is, in part, attributed to the chaperone activity associated with the proteases either encoded within the same polypeptide or on separate subunits. In contrast, substrate recognition by extracellular proteases is less selective and therefore these enzymes are generally expressed as zymogens to prevent premature proteolytic activity that would be detrimental to the cell. These extracellular proteases are activated in complex cascades involving auto-processing and proteolytic maturation. Thus, proteolysis has been adopted by bacterial pathogens at multiple levels to ensure the success of the pathogen in contact with the human host.

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Acknowledgement

We would like to thank the Danish Council for Independent Research, Natural Sciences for support grant number: 272-08-0371.

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  1. Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Stigbøjlen 4, Frederiksberg, C 1870, Denmark

    Dorte Frees, Lone Brøndsted & Hanne Ingmer

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  1. Dorte Frees
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  2. Lone Brøndsted
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  3. Hanne Ingmer
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Correspondence to Hanne Ingmer .

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  1. , Department of Biochemistry, La Trobe Institute for Molecular Science, Plenty Rd, Melbourne, 3086, Victoria, Australia

    David A. Dougan

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Frees, D., Brøndsted, L., Ingmer, H. (2013). Bacterial Proteases and Virulence. In: Dougan, D. (eds) Regulated Proteolysis in Microorganisms. Subcellular Biochemistry, vol 66. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-5940-4_7

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