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Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences

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Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2314))

Abstract

Whole-genome sequencing (WGS) has shown immense value in enabling identification and characterization of bacterial taxa. This is particularly true for mycobacteria, where culture-based characterization becomes delayed by the inherently slow growth rate of these organisms. This chapter reviews the general techniques behind WGS and their optimization, existing techniques for species-level identification and the advantages of WGS for this purpose, and a variety of useful tools for the genomic characterization of mycobacterial strains.

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Authors and Affiliations

  1. ATCC, BEI Resources, Manassas, VA, USA

    Marco A. Riojas & Manzour Hernando Hazbón

  2. ATCC, Manassas, VA, USA

    Andrew M. Frank, Samuel R. Greenfield & Stephen P. King

  3. University of Bradford, Bradford, UK

    Conor J. Meehan

  4. Department of Medicine, National Jewish Health, Denver, CO, USA

    Michael Strong

  5. University of Virginia, Charlottesville, VA, USA

    Alice R. Wattam

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  1. Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA, USA

    Tanya Parish

  2. Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA, USA

    Anuradha Kumar

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Riojas, M.A. et al. (2021). Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences. In: Parish, T., Kumar, A. (eds) Mycobacteria Protocols. Methods in Molecular Biology, vol 2314. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1460-0_19

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