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Observation of Parkin-Mediated Mitophagy in Pancreatic β-Cells

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Mitophagy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1759))

Abstract

Mitophagy is a cellular process of autophagy-based mitochondrial degradation that eliminates dysfunctional mitochondria and ensures cellular homeostasis. In pancreatic islet β-cells, mitochondria play a pivotal role in glucose-stimulated insulin secretion through ATP production from glucose oxidation. Recent studies have shown that impaired mitophagy and the subsequent mitochondrial compromise contribute to β-cell dysfunction and glucose intolerance. In this chapter, we describe a protocol to monitor Parkin-mediated mitophagy in pancreatic MIN6 β-cells using flow cytometry and a pH-sensitive fluorophore, mKeima.

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Acknowledgements

This study was supported in part by Grants-in-Aid from the Ministry of Education, Science and Culture of Japan (MEXT) (KAKENHI 15K09144 to SM).

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Authors and Affiliations

  1. Department of Cardiovascular Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho Kamigyo-ku, Kyoto, Japan

    Atsushi Hoshino & Satoaki Matoba

Authors
  1. Atsushi Hoshino
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  2. Satoaki Matoba
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Corresponding author

Correspondence to Satoaki Matoba .

Editor information

Editors and Affiliations

  1. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Nobutaka Hattori

  2. Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Shinji Saiki

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Hoshino, A., Matoba, S. (2017). Observation of Parkin-Mediated Mitophagy in Pancreatic β-Cells. In: Hattori, N., Saiki, S. (eds) Mitophagy. Methods in Molecular Biology, vol 1759. Humana Press, New York, NY. https://doi.org/10.1007/7651_2017_39

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  • DOI: https://doi.org/10.1007/7651_2017_39

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7749-9

  • Online ISBN: 978-1-4939-7750-5

  • eBook Packages: Springer Protocols

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