Abstract
Live cell imaging at high resolution of pollen tubes growing in vitro requires an experimental setup that maintains the elongated cells in a single optical plane and allows for controlled exchange of growth medium. As a low-cost alternative to lithography-based microfluidics, we developed a silicone-based spacer system that allows introducing spatial features and flexible design. These growth chambers can be cleaned and reused repeatedly.
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Bertrand-Rakusová, H., Chebli, Y., Geitmann, A. (2020). Silicone Chambers for Pollen Tube Imaging in Microstructured In Vitro Environments. In: Geitmann, A. (eds) Pollen and Pollen Tube Biology. Methods in Molecular Biology, vol 2160. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0672-8_15
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DOI: https://doi.org/10.1007/978-1-0716-0672-8_15
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