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Prediction of Cellular Burden with Host–Circuit Models

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Synthetic Gene Circuits

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2229))

Abstract

Heterologous gene expression draws resources from host cells. These resources include vital components to sustain growth and replication, and the resulting cellular burden is a widely recognized bottleneck in the design of robust circuits. In this tutorial we discuss the use of computational models that integrate gene circuits and the physiology of host cells. Through various use cases, we illustrate the power of host–circuit models to predict the impact of design parameters on both burden and circuit functionality. Our approach relies on a new generation of computational models for microbial growth that can flexibly accommodate resource bottlenecks encountered in gene circuit design. Adoption of this modeling paradigm can facilitate fast and robust design cycles in synthetic biology.

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Author information

Authors and Affiliations

  1. School of Biological Sciences, University of Edinburgh, Edinburgh, UK

    Evangelos-Marios Nikolados & Diego A. Oyarzún

  2. School of Informatics, University of Edinburgh, Edinburgh, UK

    Andrea Y. Weiße & Diego A. Oyarzún

Authors
  1. Evangelos-Marios Nikolados
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  2. Andrea Y. Weiße
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  3. Diego A. Oyarzún
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Corresponding author

Correspondence to Diego A. Oyarzún .

Editor information

Editors and Affiliations

  1. School of Engineering, Institute for Bioengineering, University of Edinburgh, Edinburgh, UK

    Filippo Menolascina

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Nikolados, EM., Weiße, A.Y., Oyarzún, D.A. (2021). Prediction of Cellular Burden with Host–Circuit Models. In: Menolascina, F. (eds) Synthetic Gene Circuits . Methods in Molecular Biology, vol 2229. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1032-9_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1032-9_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1031-2

  • Online ISBN: 978-1-0716-1032-9

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