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Imaging Mitochondrial Hydrogen Peroxide in Living Cells

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Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2275))

Abstract

Hydrogen peroxide (H2O2) produced from mitochondria is intimately involved in human health and disease, but is challenging to selectively monitor inside living systems. The fluorescent probe MitoPY1 provides a practical tool for imaging mitochondrial H2O2 and has been demonstrated to function in a variety of diverse cell types. In this chapter, we describe the synthetic preparation of the small molecule probe MitoPY1 , methods for validating this probe in vitro and in live cells, and an example procedure for measuring mitochondrial H2O2 in a cell culture model of Parkinson’s disease.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Center for Drug Discovery, Design, and Delivery (CD4), Southern Methodist University, Dallas, TX, USA

    Alexander R. Lippert

  2. Department of Chemistry, The University of Chicago, Chicago, IL, USA

    Bryan C. Dickinson

  3. School of Chemistry, The University of Sydney, Sydney, NSW, Australia

    Elizabeth J. New

Authors
  1. Alexander R. Lippert
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  2. Bryan C. Dickinson
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  3. Elizabeth J. New
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Corresponding author

Correspondence to Elizabeth J. New .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutical Sciences, Midwestern University, College of Pharmacy, Glendale, AZ, USA

    Volkmar Weissig

  2. Cochin Hospital, Cochin Institute, INSERM U1016, Paris, France

    Marvin Edeas

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Lippert, A.R., Dickinson, B.C., New, E.J. (2021). Imaging Mitochondrial Hydrogen Peroxide in Living Cells. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine . Methods in Molecular Biology, vol 2275. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1262-0_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1262-0_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1261-3

  • Online ISBN: 978-1-0716-1262-0

  • eBook Packages: Springer Protocols

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