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Zygotic Embryogenesis in Flowering Plants

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Doubled Haploid Technology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2288))

Abstract

In the context of plant regeneration, in vitro systems to produce embryos are frequently used. In many of these protocols, nonzygotic embryos are initiated that will produce shoot-like structures but may lack a primary root. By increasing the auxin-to-cytokinin ratio in the growth medium, roots are then regenerated in a second step. Therefore, in vitro systems might not or only partially execute a similar developmental program as employed during zygotic embryogenesis. There are, however, in vitro systems that can remarkably mimic zygotic embryogenesis such as Brassica microspore-derived embryos. In this case, the patterning process of these haploid embryos closely follows zygotic embryogenesis and all fundamental tissue types are generated in a rather similar manner. In this review, we discuss the most fundamental molecular events during early zygotic embryogenesis and hope that this brief summary can serve as a reference for studying and developing in vitro embryogenesis systems in the context of doubled haploid production.

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Acknowledgments

We would like to thank Gerd Jürgens for comments on the manuscript and apologize to our colleagues whose work we have not included in the chapter due to our focus on Brassicaceae embryogenesis. Research in our group is supported by the German Science Foundation (Deutsche Forschungsgemeinschaft—DFG: SFB1101/B01 to M.B.), the Chinese Scholarship Council (Fellowship No. 201806320131 to Y.M.), and the Max Planck Society.

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  1. Department of Cell Biology, Max Planck Institute for Developmental Biology, Tuebingen, Germany

    Houming Chen, Yingjing Miao, Kai Wang & Martin Bayer

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  1. Houming Chen
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  2. Yingjing Miao
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  3. Kai Wang
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Correspondence to Martin Bayer .

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  1. Cell Biology Group - COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Jose M. Segui-Simarro

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Chen, H., Miao, Y., Wang, K., Bayer, M. (2021). Zygotic Embryogenesis in Flowering Plants. In: Segui-Simarro, J.M. (eds) Doubled Haploid Technology. Methods in Molecular Biology, vol 2288. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1335-1_4

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  • DOI: https://doi.org/10.1007/978-1-0716-1335-1_4

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