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In Vitro and In Vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods

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Methods and Techniques in Ethnobiology and Ethnoecology

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Abstract

Researchers have conducted tests for antimicrobial activity of natural products by several methods, and a huge collection of results has been generated. However, the lack of standardization of basic methods of investigation has led to accumulation of non-useful data. The diversity of protocols has created divergences among specialists, and often different results are obtained with the same plant extract. Although antimicrobial tests for natural products have not been standardized by regulatory agencies, in this chapter, we recommend the use of some technical parameters for susceptibility testing reviewed by the Clinical and Laboratories Standards Institute.

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Author information

Authors and Affiliations

  1. Laboratory of Microbiology and Immunology, Department of Biology, Federal Rural University of Pernambuco, Recife, Pernambuco, Brazil

    José Vitor Lima-Filho

  2. Department of Pathology and Legal Medicine, Federal University of Ceará, Fortaleza, CE, Brazil

    Rossana de Aguiar Cordeiro

Authors
  1. José Vitor Lima-Filho
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  2. Rossana de Aguiar Cordeiro
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Editor information

Editors and Affiliations

  1. Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, Pernambuco, Brazil

    Ulysses Paulino Albuquerque

  2. Departmento de Ciências Biológicas, Universidade Católica de Pernambuco, Recife, Pernambuco, Brazil

    Luiz Vital Fernandes Cruz da Cunha

  3. Departamento de Fitotecnia e Ciências Ambientais, Universidade Federal da Paraíba, Areia, Paraíba, Brazil

    Reinaldo Farias Paiva de Lucena

  4. Departamento de Biologia, Universidade Estadual da Paraiba, Campina Grande, Paraíba, Brazil

    Rômulo Romeu Nobrega Alves

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Lima-Filho, J.V., de Aguiar Cordeiro, R. (2014). In Vitro and In Vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods. In: Albuquerque, U., Cruz da Cunha, L., de Lucena, R., Alves, R. (eds) Methods and Techniques in Ethnobiology and Ethnoecology. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-8636-7_17

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  • DOI: https://doi.org/10.1007/978-1-4614-8636-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-8635-0

  • Online ISBN: 978-1-4614-8636-7

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