Abstract
The fabrication of three-dimensional (3D) constructs relies heavily on the use of biomaterial-based scaffolds. These are required as mechanical supports as well as to translate two-dimensional cultures to 3D cultures for clinical applications. Regardless of the choice of scaffold, timely degradation of scaffolds is difficult to achieve and undegraded scaffold material can lead to interference in further tissue development or morphogenesis. In cartilage tissue engineering, hydrogel is the highly preferred scaffold material as it shares many similar characteristics with native cartilaginous matrix. Hence, we employed gelatin microspheres as porogens to create a microcavitary alginate hydrogel as an interim scaffold to facilitate initial chondrocyte 3D culture and to establish a final scaffold-free living hyaline cartilaginous graft (LhCG) for cartilage tissue engineering.
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References
Gong Y, Su K, Lau TT et al (2010) Microcavitary hydrogel-mediating phase transfer cell culture for cartilage tissue engineering. Tissue Eng 16:3611–3622
Su K, Lau TT, Leong W et al (2012) Creating a living hyaline cartilage graft free from non-cartilaginous constituents: an intermediate role of a biomaterial scaffold. Adv Funct Mater 22:972–978
Masuda K, Sah RL, Hejna MJ et al (2003) A novel two step method for the formation of tissue engineered cartilage by mature bovine chondrocytes: the alginate recovered chondrocyte (ARC) method. J Orthop Res 21:139–148
Leong W, Lau TT, Wang D-A (2013) A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system. Acta Biomater 9:6459–6467
Lau TT, Peck Y, Huang W et al (2014) Optimization of chondrocyte isolation and phenotype characterization for cartilage tissue engineering. Tissue Eng Part C Method. doi:10.1089/ten.TEC.2014.0159
Acknowledgement
This work was supported by AcRF Tier 1 RG 36/12 and AcRF Tier 2 ARC 1/13, Ministry of Education, Singapore.
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Lau, T.T., Leong, W., Peck, Y., Su, K., Wang, DA. (2015). Use of Interim Scaffolding and Neotissue Development to Produce a Scaffold-Free Living Hyaline Cartilage Graft. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_10
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DOI: https://doi.org/10.1007/978-1-4939-2938-2_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2937-5
Online ISBN: 978-1-4939-2938-2
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