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Existence ofSinorhizobium meliloti genomic fragment hybridizing with thenifM gene ofKlebsiella pneumoniae

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Abstract

A novel finding that genomic restriction fragments of symbiotic nitrogen fixerS. meliloti hybridized withnifM gene probe of the free-living diazotrophKlebsiella pneumoniae is reported. WhenSmaI endonuclease was used to digestS. meliloti DNA, a unique hybridizing band was obtained.

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References

  • Burgess B.K.: pp. 103–104 in C. Veeger, W.E. Newton (Eds):Advances in Nitrogen Fixation Research. Nijhoff/Junk, The Hague 1984.

    Google Scholar 

  • Evans D., Jones R., Woodley P., Wilborn R., Robson R.: Nucleotide sequence and genetic analysis of theAzotobacter chroococcum nifUSVWZM gene cluster, including a new gene (nifP) which encodes a serine acetyl-transferase.J.Bacteriol. 173, 5457–5469 (1991).

    PubMed  CAS  Google Scholar 

  • Jacobson M.R., Cash V.L., Weiss M.C., Laird N.F., Newton W.E., Dean D.R.: Biochemical and genetic analysis of thenifUSVWZM cluster fromAzotobacter vinelandii.Mol.Gen.Genet. 219, 49–57 (1989).

    Article  PubMed  CAS  Google Scholar 

  • Masterson R.V., Prakash R.K., Atherly A.G.: Conservation of symbiotic nitrogen fixation gene sequences inRhizobium japonicum andBradyrhizobium japonicum.J.Bacteriol. 163, 21–26 (1985).

    PubMed  CAS  Google Scholar 

  • Mortenson L.E., Thorneley R.N.F.: Structure and function of nitrogenase.Ann.Rev.Biochem. 48, 387–418 (1979).

    Article  PubMed  CAS  Google Scholar 

  • Orosz L., Svab Z., Kondorosi A., Sik T.: Genetic studies onRhizophage 16-3. Genes and functions on the chromosome.Mol.Gen.Genet. 125, 341–350 (1973).

    PubMed  CAS  Google Scholar 

  • Paul W., Merrick M.: The nucleotide sequence of thenifM gene ofKlebsiella pneumoniae and identification of a newnif gene:nifZ.Eur.J.Biochem. 170, 259–265 (1987).

    Article  PubMed  CAS  Google Scholar 

  • Paul W., Merrick M.: The roles of thenifW, nifZ andnifM genes ofKlebsiella pneumoniae in nitrogenase biosynthesis.Eur.J.Biochem. 178, 675–682 (1989).

    Article  PubMed  CAS  Google Scholar 

  • Petrova N., Gigova L., Venkov P.: NifH and NifM proteins interact as demonstrated by the yeast two-hybrid system.Biochem. Biophys.Res.Commun. 270, 863–867 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Petrova N., Gigova L., Venkov P.: Dimerization ofRhizobium meliloti NifH protein inSaccharomyces cerevisiae cells requires simultaneous expression of NifM protein.Internat.J.Biochem.Cell Biol. 34, 33–42 (2002).

    Article  CAS  Google Scholar 

  • Sambrook J., Fritsch E.F., Maniatis T.:Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1989.

    Google Scholar 

  • Selenska-Trajkova S., Radeva G., Markov K.: Comparison betweenRhizobium galegae andRhizobium meliloti plasmid contents.Lett.Appl.Microbiol. 10, 123–126 (1990).

    Article  Google Scholar 

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Correspondence to L. Gigova.

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This work was supported byBulgarian Academy of Sciences.

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Gigova, L., Petrova, N., Dimova, S. et al. Existence ofSinorhizobium meliloti genomic fragment hybridizing with thenifM gene ofKlebsiella pneumoniae . Folia Microbiol 52, 447–448 (2007). https://doi.org/10.1007/BF02932102

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  • DOI: https://doi.org/10.1007/BF02932102

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