Abstract
The hot spring water of Atri in India was believed to have disease curing property. An antibacterial producing organism was isolated and identified as Bacillus paralicheniformis by morphology, microscopy, and 16S-rRNA. Its secretion inhibited bacteria, yeast, and fungus in well-diffusion-method. The secreted antimicrobial was a 16.74 kDa protein homologous of chicken-lysozyme-C. The novel lysozyme’s activities were recorded under different parameters. It was active from pH 5–9 and endured up to 60 °C for 120 min. Complete cell wall lysis of S. flexneri and P. aeruginosa was observed under a microscope at 4500× with a minimum inhibitory concentration of 7.8 µg/ml, while others required a higher dose, i.e., 13 µg/ml, and 20 µg/ml for E.coli and S. typhimurium, respectively. The discovered lysozyme has the extraordinary potential to lyse Gram-positive bacteria, yeast, fungus, and more efficiently lyse chick-lysozyme-C resistant lipopolysaccharide rich Gram-negative bacteria's outer cell wall.
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Acknowledgements
The authors acknowledge the kind cooperation of Dr. Sujay Singh, Director, Imgenex India Private Limited, Bhubaneswar. The research facility was provided by the Centre of Excellence (OHEPEE), Ravenshaw University, Cuttack. Bacterial strains were provided by Kalinga Institute of Industrial Technology, Bhubaneswar and Institute of Life Sciences, Bhubaneswar. I express my deep sense of gratitude to Protein Lounge group, Imgenex India Pvt. Ltd. I am also grateful to Mrs. Himadri Tanaya Behera, Mr. Sanjeet Kumar Das, Mr. Abhik Mazumdar and Mr. Mahendra Kumar Lenka, Imgenex India Pvt. Ltd. for assisting in performing different experiments. Lastly I would like to express my indebtedness to my wife and parents for providing me moral support and encouragement.
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KKS, and AKP: Conceived and designed the experiments; PO, NPK, AKP, SN, and KKS: Performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, and wrote the paper.
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Ojha, P., Kar, N.P., Nayak, S. et al. Isolation of a broad spectrum antimicrobial producing thermophilic Bacillus and characterization of its antimicrobial protein. Arch Microbiol 203, 2059–2073 (2021). https://doi.org/10.1007/s00203-020-02162-w
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DOI: https://doi.org/10.1007/s00203-020-02162-w