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relA and spoT Gene Expression is Modulated in Salmonella Grown Under Static Magnetic Field

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Abstract

Virtually all bacterial species synthesize high levels of (p)ppGpp (guanosine penta- or tetraphosphate), a pleiotropic regulator of the stringent response and other stresses in bacteria. relA and spoT genes are, respectively, involved in synthesis and synthesis/biodegradation of (p)ppGpp. We aimed in this work to evaluate the impact of static magnetic field (SMF) 200 mT exposure on the expression of relA and spoT genes in Salmonella enterica Hadar. Bacteria were exposed to a SMF during 9 h, and RNA extraction was followed by reverse transcriptase polymerase chain reaction (RT-PCR). The relative quantification of mRNA expression levels using the 16S rRNA reference gene did not change during the SMF exposure. However, results showed a significant increase in gene expression for relA after 3 h of exposure (P < 0.05) and after 6 h for spoT (P < 0.05). The differential gene expression of relA and spoT could be considered as a potential stress response to a SMF exposure in Salmonella related to the production/degradation of (p)ppGpp.

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Acknowledgements

This work was financially supported by the Ministry of Higher Education and Scientific Research (Tunisia).

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  1. Laboratoire de Biochimie et Biologie Moléculaire, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisia

    Alya El May, Jihen Zouaoui, Sarra Snoussi, Ramla Ben Mouhoub & Ahmed Landoulsi

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  1. Alya El May
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  2. Jihen Zouaoui
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AEM—Planning, executing analysis of experiments, writing, and review of manuscript. JZ—Executing and analysis of experiments. SS—Technical support. RBM—Analysis of experiments.AL—Supervision, planning, and resources.

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Correspondence to Alya El May.

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All the cited authors contributed to the generation or analysis of the data,, and they do not have any conflict of interest. No competing financial interests exist.

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El May, A., Zouaoui, J., Snoussi, S. et al. relA and spoT Gene Expression is Modulated in Salmonella Grown Under Static Magnetic Field. Curr Microbiol 78, 887–893 (2021). https://doi.org/10.1007/s00284-021-02346-7

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  • DOI: https://doi.org/10.1007/s00284-021-02346-7

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