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Antifungal Activity of Serratia rubidaea Mar61-01 Purified Prodigiosin Against Colletotrichum nymphaeae, the Causal Agent of Strawberry Anthracnose

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Abstract

Strawberry anthracnose, caused by Colletotrichum nymphaeae is an important disease of strawberry. In this study, an endophytic bacterium isolated from the stem of Fragaria × ananassa cv Paros and identified as Serratia rubidaea strain Mar61-01 based on phenotypic, biochemical characteristics and molecular phylogenetic analysis. Antagonistic activities of this endophytic bacterium against C. nymphaeae were investigated under in vitro, in vivo and greenhouse conditions. The strain Mar61-01 reduced mycelial growth and conidial germination of C. nymphaeae in in vitro tests. Furthermore, it reduced disease severity on inoculated strawberry fruits and seedlings compared with uninoculated control. In addition, the strain Mar61-01 produced prodigiosin pigment. The pigment was purified by thin layer chromatography and its chemical structure was characterized by FT-IR and NMR (400 MHz) spectra. The results indicated that prodigiosin is a key feature in biocontrol of C. nymphaeae. The inhibition growth of C. nymphaeae under in vitro, in vivo, and greenhouse conditions showed that S. rubidaea Mar61-01 has the potential for managing of strawberry anthracnose.

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Acknowledgements

This research was supported by the Plant Protection Department of University of Kurdistan (Grant No: 96/19/30039).

Funding

This research project was funded by University of Kurdistan.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

    Zahra Alijani & Jahanshir Amini

  2. Department of Biology and Biotechnology, Faculty of Sciences, University of Kurdistan, Sanandaj, Iran

    Morahem Ashengroph

  3. Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

    Bahman Bahramnejad

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  1. Zahra Alijani
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  2. Jahanshir Amini
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  3. Morahem Ashengroph
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  4. Bahman Bahramnejad
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ZA: acquisition of data, visualization, writing—original draft, researcher. JA: conceptualization, project administration, funding acquisition. MA: formal analysis, writing—reviewing & editing. BB: advisor, resources.

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Correspondence to Jahanshir Amini.

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Alijani, Z., Amini, J., Ashengroph, M. et al. Antifungal Activity of Serratia rubidaea Mar61-01 Purified Prodigiosin Against Colletotrichum nymphaeae, the Causal Agent of Strawberry Anthracnose. J Plant Growth Regul 41, 585–595 (2022). https://doi.org/10.1007/s00344-021-10323-4

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