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The impact of the sepsis on female urogenital system: the role of pregabalin

  • Gynecologic Endocrinology and Reproductive Medicine
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Abstract

Purpose

The aim of the study was to investigate the oxidative damage and inflammatory effects of sepsis on the urogenital system in the Lipopolysaccharide (LPS)-induced sepsis model and ameliorating role of Pregabalin (PGB).

Methods

Twenty-four female Wistar Albino rats (12 months old) were divided into 3 groups as follows: Sepsis group (Group S) (5 mg/kg LPS, i.p, single dose); Sepsis+ PGB group (Group SP) (5 mg/kg LPS, i.p, single dose and 30 mg/kg PGB); Control group (Group C) (0.1 ml/oral and i.p. saline, single dose), 6 h after LPS administration, the animals were killed. Subsequently, analyses of urogenital tissue oxidant/antioxidant status, histopathological and immunohistochemical analyses were performed.

Results

Total oxidative status (TOS) and oxidative stress index (OSI) values in the urogenital tissues were increased in Group S (Total anti-oxidative status (TAS) decreased) compared to the Control group (p < 0.05). PGB improved these values (p < 0.05). The immunohistochemical markers [Caspase-3, granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), Serum Amyloid A (SAA) and inducible nitric oxide synthase (iNOS)] were significantly increased in Group S except for bladder (p < 0.001). Statistically significant immunohistochemical positiveness was found only for IL-6 in urinary bladder, though all the others values were negative. With the administration of PGB (Group SP), the expressions of these immunoreactions were markedly decreased (p < 0.001).

Conclusions

These findings demonstrated that sepsis caused oxidative stress and inflammation in the urogenital tissues. We have revealed that PGB ameliorated tissue damage caused by sepsis.

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Acknowledgements

The authors thank the participants of this study.

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Gynaecology and Obstetrics, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkey

    Ilker Gunyeli

  2. Department of Physiology, Faculty of Medicine, Suleyman Demirel University Isparta, Isparta, Turkey

    Mustafa Saygin

  3. Department of Pathology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey

    Ozlem Ozmen

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Contributions

GI contributed in the literature search, project development, manuscript writing/editing, data collection and interpretation, experimental rat study, rat killing and revised the article; SM contributed in biochemical analysis, statistical analysis, rat killing; OO contributed in histopathological and immunohistochemical analyses, management data analysis, and statistical analysis.

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Correspondence to Ilker Gunyeli.

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Gunyeli, I., Saygin, M. & Ozmen, O. The impact of the sepsis on female urogenital system: the role of pregabalin. Arch Gynecol Obstet 300, 1067–1082 (2019). https://doi.org/10.1007/s00404-019-05285-8

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