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Novel conserved segments are associated with differential expression patterns for Pinaceae dehydrins

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Abstract

Dehydrins are thought to play an essential role in the response, acclimation and tolerance to different abiotic stresses, such as cold and drought. These proteins have been classified into five groups according to the presence of conserved and repeated motifs in their amino acid sequence. Due to their putative functions in the response to stress, dehydrins have been often used as candidate genes in studies on population variability and local adaptation to environmental conditions. However, little is still known regarding the differential role played by such groups or the mechanism underlying their function. Based on the sequences corresponding to dehydrins available in public databases we have isolated eight different dehydrins from cDNA of Pinus pinaster. We have obtained also their genomic sequences and identified their intron/exon structure. Quantitative RT-PCR analysis of their expression pattern in needles, stems and roots during a severe and prolonged drought stress, similar to the ones trees must face in nature, is also reported. Additionally, we have identified two amino acid motifs highly conserved and repeated in Pinaceae dehydrins and absent in angiosperms, presumably related to the divergent expression profiles observed.

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Abbreviations

EST:

Expressed sequence tag

LEA proteins:

Late embryogenesis abundant proteins

qRT-PCR:

Quantitative reverse transcription polymerase chain reaction

TC:

Tentative contig

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Acknowledgments

The authors would like to thank Dr. Luis Gil from UPM for technical and scientific support. We also thank Dr. Jesús Rodríguez-Calcerrada and three anonymous reviewers for their helpful comments and suggestions. This work has been funded through the projects AGL2006-03242/FOR (Spanish Ministry of Education and Science), CCG07-UPM/AMB-1932 and CCG10-UPM/AMB-5038 (Madrid Regional Government–UPM). PP has a pre-doctoral fellowship from the Spanish Ministry of Education and Science.

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Authors and Affiliations

  1. GENFOR Grupo de investigación en Genética y Fisiología Forestal, Universidad Politécnica de Madrid, 28040, Madrid, Spain

    Pedro Perdiguero, M. Carmen Barbero, Álvaro Soto & Carmen Collada

  2. Unidad Mixta de Genómica y Ecofisiología Forestal, INIA/UPM, Madrid, Spain

    Pedro Perdiguero, M. Carmen Barbero, M. Teresa Cervera, Álvaro Soto & Carmen Collada

  3. Departamento de Genética y Fisiología Forestal, CIFOR-INIA, Madrid, Spain

    M. Teresa Cervera

Authors
  1. Pedro Perdiguero
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  2. M. Carmen Barbero
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  3. M. Teresa Cervera
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  4. Álvaro Soto
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  5. Carmen Collada
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Correspondence to Álvaro Soto.

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Supplementary material 1 (DOC 36 kb)

425_2012_1737_MOESM2_ESM.pdf

Supplementary material Supplementary Figure S1 Alignment of amino acid sequences corresponding to gymnosperm and angiosperm dehydrins available in public databases. Amino acid sequences deduced from ESTs are labelled with an asterisk and in some cases are incomplete. Conserved motifs (A, E, Y, S, K) are highlighted, as well as differentially abundant residues (E, P v. G. T). See text for details (PDF 644 kb)

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Perdiguero, P., Barbero, M.C., Cervera, M.T. et al. Novel conserved segments are associated with differential expression patterns for Pinaceae dehydrins. Planta 236, 1863–1874 (2012). https://doi.org/10.1007/s00425-012-1737-4

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