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Cell proliferation in the central nervous system of an adult semiterrestrial crab

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Abstract

Neurogenesis occurs in adults of most organisms, both vertebrates and invertebrates. In semiterrestrial crabs of the infraorder Brachyura, the deutocerebrum, where neurogenesis occurs, processes the olfactory sensory information from the antennae. The deutocerebrum is composed of a pair of olfactory lobes associated with cell clusters 9 and 10 (Cl 9 and Cl 10), containing proliferating cells. Because the location of the neurogenic niche in brachyuran semiterrestrial crabs has not been defined, here we describe a neurogenic niche in the central olfactory system of the crab Ucides cordatus and report two types of glial cells in the deutocerebrum, based on different markers. Serotonin (5-hydroxytryptamine) labeling was used to reveal neuroanatomical aspects of the central olfactory system and the neurogenic niche. The results showed a zone of proliferating neural cells within Cl 10, which also contains III beta-tubulin (Tuj1)+ immature neurons, associated with a structure that has characteristics of the neurogenic niche. For the first time, using two glial markers, glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS), we identified two types of astrocyte-like cells in different regions of the deutocerebrum. This study adds to the understanding of neurogenesis in a brachyuran semiterrestrial crustacean and encourages comparative studies between crustaceans and vertebrates, including mammals, based on shared aspects of both mechanisms of neurogenesis and regenerative potentials.

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Abbreviations

AL:

Accessory lobe

BrdU:

5-Bromo-2′-deoxyuridine

Cl 9:

Cluster 9

Cl 10:

Cluster 10

DAPi:

4′,6-Diamidino-2-phenylindole

DGN:

Dorsal-giant neurons

EM:

External medulla

GFAP:

Glial fibrillary acidic protein

GS:

Glutamine synthetase

HB:

Hemiellipsoid body

IM:

Internal medulla

5HT:

5-Hydroxytryptamine (serotonin)

L:

Lamina

NeuN:

Neuronal nuclei

OGT:

Olfactory globular tract

OL:

Olfactory lobe

ORNs:

Olfactory receptor neurons

PBS:

Phosphate buffer saline

PH3:

Phospho-histone H3

PF:

Paraformaldehyde

PI:

Propidium iodide

TM:

Terminal medulla

Tuj1:

III beta-tubulin

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Funding

This study was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Biológicas - Fisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, RJ, 21941-590, Brazil

    Gabriela Hollmann, Rafael Linden & Silvana Allodi

  2. Programa de Pós-Graduação em Biologia Celular e do Desenvolvimento, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina-UFSC, João Pio Duarte Silva, 241, Florianópolis, SC, 88037-000, Brazil

    Gabriela Hollmann

  3. Programa de Pós-Graduação em Ciências Biológicas - Biofísica , Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro-UFRJ , 21941-590, Rio de Janeiro, Brazil

    Paula Grazielle Chaves da Silva, Rafael Linden & Silvana Allodi

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  1. Gabriela Hollmann
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  2. Paula Grazielle Chaves da Silva
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  3. Rafael Linden
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  4. Silvana Allodi
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Correspondence to Gabriela Hollmann.

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The authors declare that they have no conflict of interest.

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All applicable international, national and/or institutional guidelines for the care and use of animals were followed. All procedures adopted in this study, including the location where the animals were captured, were performed after approval by the National Environmental Committee (Certificate # 14689-1/IBAMA/2008, permission to use the animals # 2440408) and by the Ethics Commission on Research Animals of the Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro (protocol DHEICB 005). This article does not contain any studies with human participants performed by any of the authors.

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Hollmann, G., da Silva, P.G.C., Linden, R. et al. Cell proliferation in the central nervous system of an adult semiterrestrial crab. Cell Tissue Res 384, 73–85 (2021). https://doi.org/10.1007/s00441-021-03413-y

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