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Biotic nitrogen fixation in the bryosphere is inhibited more by drought than warming

  • Global change ecology – original research
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Abstract

The boreal forest is of particular interest to climate change research due to its large circumpolar distribution and accumulated soil carbon pool. Carbon uptake in this ecosystem is nitrogen (N)-limited, therefore factors affecting carbon or nitrogen dynamics in the boreal forest can have consequences for global climate. We used a 2-year field experiment to investigate the response of biotic nitrogen fixation by cyanobacteria associated with boreal forest bryophytes, in a factorial experiment combining simulated climate change with habitat fragmentation treatments. We simulated climate change conditions using open-top greenhouse chambers in the field, which increased mean and maximum temperatures, and created a precipitation gradient from ambient levels in the center to extreme drought conditions at the periphery of the chamber. The dry patches near the chamber walls exhibited almost no N-fixation, despite having similar densities of cyanobacteria (predominantly Stigonema sp.) as other patches. Rates of N-fixation were best explained by a model containing moisture, fragmentation, cyanobacteria density and time; warming was not a significant variable affecting N-fixation. There was no significant interaction between warming and fragmentation. These results suggest that cyanobacteria responded physiologically to drought by reducing N-fixation activity long before any changes in density. Ecosystem processes, such as N-fixation, can respond in the short term to environmental change much more rapidly than changes in the underlying community structure. Such rapid physiological responses may occur faster than demographic insurance effects of biodiversity.

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Acknowledgments

This project was supported by a Post-Graduate Scholarship from the Natural Sciences and Engineering Research Council of Canada (NSERC) to JAW, with a Natural Sciences and Engineering Research Council discovery grant and Canada Research Chair funding to AG. Z. Lindo helped with field work and provided friendly review during manuscript preparation. O. Choulik provided logistical support, and many nourishing meals, in Schefferville. M. Mehta helped with cyanobacteria data collection. J. Connolly provided helpful advice on mixed modeling approaches. The manuscript was greatly improved by constructive comments from three reviewers.

Author contribution statement

JAW and AG conceived and designed the experiment. JAW conducted fieldwork, collected and processed samples, and analyzed the data; JAW and AG analyzed the temperature data. JAW wrote the manuscript, AG provided editorial advice.

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  1. Department of Biology, McGill University, 1205 Docteur Penfield, Montreal, QC, H3A 1B1, Canada

    Jonathan A. Whiteley & Andrew Gonzalez

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  1. Jonathan A. Whiteley
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Correspondence to Jonathan A. Whiteley.

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Communicated by Amy Austin.

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Whiteley, J.A., Gonzalez, A. Biotic nitrogen fixation in the bryosphere is inhibited more by drought than warming. Oecologia 181, 1243–1258 (2016). https://doi.org/10.1007/s00442-016-3601-x

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