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Biosynthesis of glyceride glycoside (nonionic surfactant) by amylosucrase, a powerful glycosyltransferase

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Abstract

Amylosucrase (ASase, E.C. 2.4.1.4) is a powerful transglycosylation enzyme that can transfer glucose from sucrose to the hydroxyl (-OH) group of various compounds. In this study, recombinant ASases from Deinococcus geothermalis (DgAS) and Bifidobacterium thermophilum (BtAS) were used to synthesize biosurfactants based on the computational analysis of predicted docking simulations. Successful predictions of the binding affinities, conformations, and three-dimensional structures of three surfactants were computed from receptor-ligand binding modes. DgAS and BtAS were effective in the synthesis of biosurfactants from glyceryl caprylate, glyceryl caprate, and polyglyceryl-2 caprate. The results of the transglycosylation reaction were consistent for both ASases, with glyceryl caprylate acceptor showing the highest concentration, as confirmed by thin layer chromatography. Furthermore, the transglycosylation reactions of DgAS were more effective than those of BtAS. Among the three substrates, glyceryl caprylate glycoside and glyceryl caprate glycoside were successfully purified by liquid chromatography–mass spectrometry (LC–MS) with the corresponding molecular weights.

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Acknowledgements

This paper was supported by research funds provided to newly appointed professors of Jeonbuk National University in 2019.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea

    Ye-Jin Kim, Young-Rok Kim & Cheon-Seok Park

  2. Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Inonge Noni Siziya & Dong-Ho Seo

  3. Research Group of Healthcare, Korea Food Research Institute, Wanju, 55365, Republic of Korea

    Seungpyo Hong

  4. Healthcare Research Institute, Kolon Industries, Inc, Seoul, 07793, Republic of Korea

    Gil-Yong Lee

  5. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

  6. Department of Food Science and Biotechnology, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul, 05006, Republic of Korea

    Sang-Ho Yoo

  7. Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Dong-Ho Seo

  8. Institute of Agricultural Science and Technology, Jeonbuk National University, Jeonju, 54896, Republic of Korea

    Dong-Ho Seo

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  1. Ye-Jin Kim
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Correspondence to Dong-Ho Seo.

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Kim, YJ., Siziya, I.N., Hong, S. et al. Biosynthesis of glyceride glycoside (nonionic surfactant) by amylosucrase, a powerful glycosyltransferase. Food Sci Biotechnol 30, 267–276 (2021). https://doi.org/10.1007/s10068-020-00861-0

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  • DOI: https://doi.org/10.1007/s10068-020-00861-0

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