Abstract
We sought to elucidate how the local administration of mepivacaine hydrochloride and vasopressin via the tail affects the peripheral blood flow volume, tissue dynamics, and mepivacaine’s anesthetic effect in mice. Two-hundred and twenty-six male ICR mice were used in this study. Blood flow was measured after administering mepivacaine alone or mepivacaine with either 0.03, 0.3, or 3.0 U/mL vasopressin or 10 µg/mL epinephrine via the tail tissue. The tail tissue and blood dynamics were measured using 3H-labeled mepivacaine hydrochloride with vasopressin or epinephrine. The compound nerve action potential (CNAP) was measured to clarify the anesthetic effect after administering mepivacaine with 0.3 U/mL vasopressin. The statistical methods employed were Steel–Dwass test, Mann–Whitney U test, Dunnett’s test, and Tukey test. P < 0.05 indicated statistical significance. The results revealed that the local administration of ≥ 0.03 U/mL vasopressin reduced local blood flow and prolonged 3H-M localization in the tail tissue in a concentration-dependent manner. Addition of 0.3 U/mL vasopressin enhanced and prolonged the anesthetic effect of mepivacaine. The findings suggest that adding vasopressin to a local anesthetic regimen may be effective, and thus it could be applied as a vasoconstrictor.
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References
Altura BM, Hershey SG, Zweifach BW. Effects of a synthetic analogue of vasopressin on vascular smooth muscle. Proc Soc Exp Biol Med. 1965;119:258–61.
Fleury CA, Andreo VC, Lomba PC, Dionísio TJ, Amaral SL, Santos CF, Faria FA. Comparison of epinephrine and felypressin pressure effects in 1K1C hypertensive rats treated or not with atenolol. J Anesth. 2015;29:56–64.
Cecanho R, De Luca Jr LA, Ranali J. Cardiovascular effects of felypressin. Anesth Prog. 2006;53:119–25.
Inagawa M, Ichinohe T, Kaneko Y. Felypressin, but not epinephrine, reduces myocardial oxygen tension after an injection of dental local anesthetic solution at routine doses. J Oral Maxillofac Surg. 2010;68:1013–7.
Murata N, Sunada K, Hashimoto S. Effect of adding vasopressin on the distribution of lidocaine in tissues, anesthetic action, and circulatory dynamics. Odontology. 2019. https://doi.org/10.1007/s10266-019-00449-z.
Kerschner JE, Futran ND. The effect of topical vasodilating agents on microvascular vessel diameter in the rat model. Laryngoscope. 1996;106:1429–33.
Altura BM, Altura BT. Effects of local anesthetics, antihistamines, and glucocorticoids on peripheral blood flow and vascular smooth muscle. Anesthesiology. 1974;41:197–21414.
Krejcí I, Kupková B, Vávra I, Rudinger I. Actions of neurohypophysial hormone analogues on perfused isolated rat caudal artery. Eur J Pharmacol. 1970;13:65–75.
Greene TK, Schiviz A, Hoellriegl W, Poncz M, Muchitsch EM. Animal models subcommittee of the scientific and standardization committee of the ISTH. Towards a standardization of the murine tail bleeding model. J Thromb Haemost. 2010;8:2820–2.
Ruchoux MM, Domenga V, Brulin P, Maciazek J, Limol S, Tournier-Lasserve E, Joutel A. Transgenic mice expressing mutant Notch3 develop vascular alterations characteristic of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy. Am J Pathol. 2003;162:329–42.
Kura N, Fujikawa T, Tochikubo O. New finger-occlusion plethysmograph for estimating peripheral blood flow and vascular resistance. Circ J. 2008;72:1329–35.
Hashimoto S, Yamashiro M, Fujita K, Yasuda A, Sunada K. Effects of epinephrine on lidocaine pharmacokinetics and blood volume in the dental pulp. J Endod. 2014;40:1370–4.
Ono Y, Takeuchi Y, Hisanaga N. A comparative study on the toxicity of n-hexane and its isomers on the peripheral nerve. Int Arch Occup Environ Health. 1981;48:289–94.
Weil C, Santangelo C, Welham FS, Yackel RF. Clinical evaluation of mepivacaine hydrochloride by a new method. J Am Dent Assoc. 1961;63:26–322.
Ahlquist RP. The action of various drugs on the arterial blood flow of the pregnant, canine uterus. J Am Pharm Assoc Am Pharm Assoc. 1950;39:370–3.
Landesman R, Mendelsohn B. The uterine omentum of the rat and its response to vasoconstrictor drugs. Am J Obstet Gynecol. 1956;72:84–92.
Waisvisz EL. In vitro experiments with the intact human uterus. II. Indications for the existence of 2 contractile systems in the human uterus in vitro. Ned Tijdschr Verloskd Gynaecol. 1965;65:409–18.
Shimanuki H, Takeuchi H, Kitade M, Kikuchi I, Kumakiri J, Kinoshita K. The effect of vasopressin on local and general circulation during laparoscopic surgery. J Minim Invas Gynecol. 2006;13:190–4.
Luciano AA. Myomectomy. Clin Obstet Gynaecol. 2009;52:362–71.
Song T, Kim MK, Kim ML, Jung YW, Yun BS, Seong SJ. Use of vasopressin vs epinephrine to reduce haemorrhage during myomectomy: a randomized controlled trial. Eur J Obstet Gynecol Reprod Biol. 2015;195:177–81.
Frederick J, Fletcher H, Simeon D, Mullings A, Hardie M. Intramyometrial vasopressin as a haemostatic agent during myomectomy. Br J Obstet Gynaecol. 1994;101:435–7.
Li YT, Yin CS, Chen FM, Chao TC. A useful technique for the control of severe cesarean hemorrhage: report of three cases. Chang Gung Med J. 2002;25:548–52.
Kato S, Tanabe A, Kanki K, Suzuki Y, Sano T, Tanaka K, Fujita D, Terai Y, Kamegai H, Ohmichi M. Local injection of vasopressin reduces the blood loss during cesarean section in placenta previa. J Obstet Gynaecol Res. 2014;40:1249–56.
Hobo R, Netsu S, Koyasu Y, Tsutsumi O. Bradycardia and cardiac arrest caused by intramyometrial injection of vasopressin during a laparoscopically assisted myomectomy. Obstet Gynecol. 2009;113:484–6.
Butala BP, Shah VR, Parikh BK, Jayaprakash J, Kalo J. Bradycardia and severe vasospasm caused by intramyometrial injection of vasopressin during myomectomy. Saud J Anaesth. 2014;8:396–8.
Ford DJ, Raj PP, Singh P, Regan KM, Ohlweiler D. Differential peripheral nerve block by local anesthetics in the cat. Anesthesiology. 1984;60:28–33.
De Jong RH, Nace RA. Nerve impulse conduction and cutaneous receptor responses during general anesthesia. Anesthesiology. 1967;28:851–5.
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We would like to thank Editage for English language editing.
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This study was funded by JSPS KAKENHI Grant Number JP19K19249.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by KK, SH and KS. The first draft of the manuscript was written by KK and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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This study was approved by the Animal Use and Care Committee of the Nippon Dental University School of Life Dentistry (Approval Number: 17-12) and was conducted in accordance with the committee’s guidelines.
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Katagiri, K., Hashimoto, S. & Sunda, K. Effect of vasopressin as a local anesthetic in mice. Odontology 108, 626–635 (2020). https://doi.org/10.1007/s10266-020-00491-2
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DOI: https://doi.org/10.1007/s10266-020-00491-2