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Pseudomonas lopnurensis sp. nov., an endophytic bacterium isolated from Populus euphratica at the ancient Ugan river

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Abstract

A Gram-stain negative, aerobic, rod-shaped, motile by a single polar flagellum, non-spore-forming bacterium, designated strain AL-54T, was isolated from the storage liquid in the stems of Populus euphratica tree at the ancient Ugan River in Xinjiang, PR China. Isolated AL-54T grew optimally at pH 7.0 and temperature 35 °C in the presence of 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence demonstrated that the isolate belonged to the genus Pseudomonas and was closely related to Pseudomonas songnenensis NEAU-ST5-5 T (97.6%), Pseudomonas zhaodongensis NEAU-ST5-21 T (97.5%), Pseudomonas alcaliphila AL15-21T (97.3%), Pseudomonas toyotomiensis HT-3T (97.3%), Pseudomonas oleovorans subsp. lubricantis RS1T (97.3%), Pseudomonas stutzeri ATCC 17588T (97.3%), Pseudomonas chengduensis CGMCC 2318T (97.2%), and Pseudomonas xanthomarina KMM 1447T (97.1%). Multilocus Sequences Analysis (MLSA) of strain AL-54T based on the three housekeeping genes, rpoB, rpoD and gyrB further confirmed the phylogenetic assignment of the isolates. The G+C content was 64.7 mol%. The DNA-DNA hybridization with P. songnenensis NEAU-ST5-5 T, P. zhaodongensis NEAU-ST5-21T, P. alcaliphila AL15-21T, P. toyotomiensis HT-3T, P. oleovorans subsp. lubricantis RS1T, P. stutzeri ATCC 17588T, P. chengduensis CGMCC 2318T and P. xanthomarina KMM 1447T revealed 44.0%, 44.7%, 60.1%, 48.7%, 49.1%, 60.1%, 58.9% and 60.2% relatedness respectively. The predominant quinone system is ubiquinone-9 (Q-9). The major components of the cellular fatty acids (>10%) were summed feature 8 (comprising C18:1 ω7c /C18:1 ω6c), summed feature 3 (comprising C16:1 ω7c /C16:1 ω6c) and C16:0. The detected major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylcholine (PC). On the basis of phenotypic data, chemotaxonomic and phylogenetic properties, strain AL-54T can consider as a novel species within the genus Pseudomonas, for which the name Pseudomonas lopnurensis sp. nov. is proposed. The type strain is AL-54T (= JCM 19136T = CCTCC AB 2013066T = NRRL B-59987T).

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. U1203101 and 31060002), the National Infrastructure of Natural Resources for Science and Technology Program of China (No. NIMR-2014-8) and the Project of Tianshan Innovation Team in Xinjiang Uygur Autonomous Region (2017D14014).

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  1. Tursunay Mamtimin and Nusratgul Anwar have contributed equally to this study and are joint first authors.

Authors and Affiliations

  1. School of Life Sciences, Lanzhou University, Lanzhou, 730000, People’s Republic of China

    Tursunay Mamtimin

  2. College of Life Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Nusratgul Anwar & Min Wu

  3. College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, 830046, People’s Republic of China

    Tursunay Mamtimin, Mehfuzem Abdurahman, Marygul Kurban, Manziram Rozahon, Hormathan Mamtimin, Buayshem Hamood & Erkin Rahman

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Contributions

T.M and N.A: responsible for the major experiments,analyze data and prepared the manuscript; M.A, M.R and M.K: isolated strain AL-54T and undertake physiological and biochemical experiments; H.M and B.H: responsible for purchasing reference strains; A.R and M.W: designed the experiments and guided the manuscript writing. All authors have read and approved the final manuscript.

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Correspondence to Erkin Rahman or Min Wu.

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The Gen Bank/EMBL/DDBJ accession numbers for the partial 16S rRNA, rpoB, rpoD and gyrB gene sequences of the strain AL-54T are MW138096, KJ577580, KJ577581 and KY008251 respectively. GenBank Accession Numbers for the whole genome sequences of strain AL-54T is JADDIX000000000.

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Mamtimin, T., Anwar, N., Abdurahman, M. et al. Pseudomonas lopnurensis sp. nov., an endophytic bacterium isolated from Populus euphratica at the ancient Ugan river. Antonie van Leeuwenhoek 114, 399–410 (2021). https://doi.org/10.1007/s10482-021-01524-8

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