Skip to main content
SpringerLink
Log in

Fucoidan isolated from the popular edible brown seaweed Sargassum fusiforme suppresses lipopolysaccharide-induced inflammation by blocking NF-κB signal pathway

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

The anti-inflammatory effect of a fucoidan with a molecular weight of 102.67 kDa isolated from an enzymatic digest of Sargassum fusiforme was investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and zebrafish. The results indicated that the fucoidan significantly and dose-dependently inhibited the production of inflammatory molecules, including tumor necrosis factor-alpha (TNF-α), nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6), as well as improved the viability of LPS-stimulated RAW 264.7 cells. Moreover, the fucoidan suppressed the expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) by regulating the nuclear factor kappa B (NF-κB) pathway in LPS-stimulated RAW 264.7 cells. Furthermore, in vivo test results suggested that the fucoidan remarkably reduced reactive oxygen species (ROS), cell death, and NO levels in LPS-stimulated zebrafish in a dose-dependent manner. Taken together, these results demonstrated that the fucoidan isolated from S. fusiforme possesses strong anti-inflammatory activities in vitro and in vivo, and could prove as an important candidate to be used to develop anti-inflammatory agents in pharmaceutical and cosmeceutical industries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Cheong SH, Yang H-W, Ko E-Y, Ahn G, Lee W, Kim D, Jeon Y-J, Kim K-N (2016) Anti-inflammatory effects of trans-1,3-diphenyl-2,3-epoxypropane-1-one in zebrafish embryos in vivo model. Fish Shellfish Immunol 50:16–20

    Article  CAS  Google Scholar 

  • Cui M, Wu J, Wang S, Shu H, Zhang M, Liu K, Liu K (2019) Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura. Int J Biol Macromol 129:377–385

    Article  CAS  Google Scholar 

  • Dong H, Zheng L, Yu P, Jiang Q, Wu Y, Huang C, Yin B (2020) Characterization and application of lignin–carbohydrate complexes from lignocellulosic materials as antioxidants for scavenging in vitro and in vivo reactive oxygen species. ACS Sustain Chem Eng 8:256–266

    Article  CAS  Google Scholar 

  • Guevara M, Tejera E, Iturralde GA, Jaramillo-Vivanco T, Granda-Albuja MG, Granja-Albuja S, Santos-Buelga C, González-Paramás AM, Álvarez-Suarez JM (2019) Anti-inflammatory effect of the medicinal herbal mixture infusion, Horchata, from southern Ecuador against LPS-induced cytotoxic damage in RAW 264.7 macrophages. Food Chem Toxicol 131:110594

    Article  CAS  Google Scholar 

  • Hwang D, Kang M-j, Jo JM, Seo BY, Park GN, Kim G-D (2019) Anti-inflammatory activity of β-thymosin peptide derived from Pacific oyster (Crassostrea gigas) on NO and PGE2 production by down-regulating NF-κB in LPS-induced RAW264.7 Macrophage Cells. Mar Drugs 17:129

    Article  CAS  Google Scholar 

  • Kang S-M, Kim K-N, Lee S-H, Ahn G, Cha S-H, Kim A-D, Yang X-D, Kang M-C, Jeon Y-J (2011) Anti-inflammatory activity of polysaccharide purified from AMG-assistant extract of Ecklonia cava in LPS-stimulated RAW 264.7 macrophages. Carbohydr Polym 85:80–85

    Article  CAS  Google Scholar 

  • Kim K-N, Ko Y-J, Yang H-M, Ham Y-M, Roh SW, Jeon Y-J, Ahn G, Kang M-C, Yoon W-J, Kim D, Oda T (2013) Anti-inflammatory effect of essential oil and its constituents from fingered citron (Citrus medica L. var. sarcodactylis) through blocking JNK, ERK and NF-κB signaling pathways in LPS-activated RAW 264.7 cells. Food Chem Toxicol 57:126–131

  • Kim E-A, Lee S-H, C-i K, Cha S-H, Kang M-C, Kang S-M, Ko S-C, Lee W-W, Ko J-Y, Lee J-H, Kang N, Oh J-Y, Ahn G, Jee YH, Jeon Y-J (2014) Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model. Carbohydr Polym 102:185–191

    Article  CAS  Google Scholar 

  • Ko S-C, Jeon Y-J (2015) Anti-inflammatory effect of enzymatic hydrolysates from Styela clava flesh tissue in lipopolysaccharide-stimulated RAW 264.7 macrophages and in vivo zebrafish model. Nutr Res Pract 9:219–226

    Article  CAS  Google Scholar 

  • Lee S-H, Ko C-I, Jee Y, Jeong Y, Kim M, Kim J-S, Jeon Y-J (2013) Anti-inflammatory effect of fucoidan extracted from Ecklonia cava in zebrafish model. Carbohydr Polym 92:84–89

    Article  CAS  Google Scholar 

  • Lee J-H, Ko J-Y, Kim E-A, Hwang E-K, Park CS, Lee J-S, Kim C-Y, Lee H-S, Kang H-K, Cha S-H, Jeon Y-J (2017) Identification and large isolation of an anti-inflammatory compound from an edible brown seaweed, Undariopsis peterseniana, and evaluation on its anti-inflammatory effect in in vitro and in vivo zebrafish. J Appl Phycol 29:1587–1596

    Article  CAS  Google Scholar 

  • Li Y, Fu X, Duan D, Xu J, Gao X (2018) Comparison study of bioactive substances and nutritional components of brown algae Sargassum fusiforme strains with different vesicle shapes. J Appl Phycol 30:3271–3283

    Article  CAS  Google Scholar 

  • Sanjeewa KKA, Jayawardena Thilina U, Kim H-S, Kim S-Y, Shanura FIP, Wang L, Abetunga DTU, Kim W-S, Lee D-S, Jeon Y-J (2019) Fucoidan isolated from Padina commersonii inhibit LPS-induced inflammation in macrophages blocking TLR/NF-κB signal pathway. Carbohydr Polym 224:115195

    Article  Google Scholar 

  • Wan P, Xie M, Chen G, Dai Z, Hu B, Zeng X, Sun Y (2019) Anti-inflammatory effects of dicaffeoylquinic acids from Ilex kudingcha on lipopolysaccharide-treated RAW264.7 macrophages and potential mechanisms. Food Chem Toxicol 126:332–342

    Article  CAS  Google Scholar 

  • Wang L, Lee W, Oh YJ, Cui RY, Ryu B, Jeon Y-J (2018a) Protective effect of sulfated polysaccharides from celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced skin damage by regulating NF-κB, AP-1, and MAPKs signaling pathways in vitro in human dermal fibroblasts. Mar Drugs 16:239–246

    Article  Google Scholar 

  • Wang L, Oh JY, Kim HS, Lee W, Cui Y, Lee HG, Kim Y-T, Ko JY, Jeon Y-J (2018b) Protective effect of polysaccharides from Celluclast-assisted extract of Hizikia fusiforme against hydrogen peroxide-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish. Int J Biol Macromol 112:483–489

    Article  CAS  Google Scholar 

  • Wang L, Park Y-J, Jeon Y-J, Ryu B (2018c) Bioactivities of the edible brown seaweed, Undaria pinnatifida: a review. Aquaculture 495:873–880

    Article  CAS  Google Scholar 

  • Wang L, Cui YR, Yang H-W, Lee HG, Ko J-Y, Jeon Y-J (2019a) A mixture of seaweed extracts and glycosaminoglycans from sea squirts inhibits α-MSH-induced melanogenesis in B16F10 melanoma cells. Fish Aquat Sci 22:11

    Article  Google Scholar 

  • Wang L, Kim HS, Oh JY, Je JG, Jeon Y-J, Ryu B (2019b) Protective effect of diphlorethohydroxycarmalol isolated from Ishige okamurae against UVB-induced damage in vitro in human dermal fibroblasts and in vivo in zebrafish. Food Chem Toxicol 110963

  • Wang L, Lee W, Cui YR, Ahn G, Jeon Y-J (2019c) Protective effect of green tea catechin against urban fine dust particle-induced skin aging by regulation of NF-κB, AP-1, and MAPKs signaling pathways. Environ Pollut 252:1318–1324

    Article  CAS  Google Scholar 

  • Wang L, Oh JY, Yang H-W, Kim HS, Jeon Y-J (2019d) Protective effect of sulfated polysaccharides from a celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish. Mar Life Sci Tech 1:104–111

    Article  Google Scholar 

  • Wang L, Jayawardena TU, Yang H-W, Lee HG, Kang M-C, Sanjeewa KKA, Oh JY, Jeon Y-J (2020a) Isolation, characterization, and antioxidant activity evaluation of a fucoidan from an enzymatic digest of the edible seaweed, Hizikia fusiforme. Antioxidants 9:363

    Article  CAS  Google Scholar 

  • Wang L, Oh JY, Jayawardena TU, Jeon Y-J, Ryu B (2020b) Anti-inflammatory and anti-melanogenesis activities of sulfated polysaccharides isolated from Hizikia fusiforme: short communication. Int J Biol Macromol 142:545–550

    Article  CAS  Google Scholar 

  • Wang L, Oh JY, Kim Y-S, Lee H-G, Lee J-S, Jeon Y-J, Ryu B (2020c) Anti-photoaging and anti-melanogenesis effects of fucoidan isolated from Hizikia fusiforme and its underlying mechanisms. Mar Drugs 18:427

    Article  CAS  Google Scholar 

  • Welsh P, Grassia G, Botha S, Sattar N, Maffia P (2017) Targeting inflammation to reduce cardiovascular disease risk: a realistic clinical prospect? Br J Pharmacol 174:3898–3913

    Article  CAS  Google Scholar 

  • Wijesinghe WAJP, Kim E-A, Kang M-C, Lee W-W, Lee H-S, Vairappan CS, Jeon Y-J (2014) Assessment of anti-inflammatory effect of 5β-hydroxypalisadin B isolated from red seaweed Laurencia snackeyi in zebrafish embryo in vivo model. Environ Toxicol Pharmacol 37:110–117

    Article  CAS  Google Scholar 

  • Williams CS, Mann M, DuBois RN (1999) The role of cyclooxygenases in inflammation, cancer, and development. Oncogene 18:7908–7916

    Article  CAS  Google Scholar 

  • Wu G-J, Shiu S-M, Hsieh M-C, Tsai G-J (2016) Anti-inflammatory activity of a sulfated polysaccharide from the brown alga Sargassum cristaefolium. Food Hydrocoll 53:16–23

    Article  CAS  Google Scholar 

  • Yang Y, Chen J, Lei L, Li F, Tang Y, Yuan Y, Zhang Y, Wu S, Yin R, Ming J (2019) Acetylation of polysaccharide from Morchella angusticeps peck enhances its immune activation and anti-inflammatory activities in macrophage RAW264.7 cells. Food Chem Toxicol 125:38–45

    Article  CAS  Google Scholar 

  • Zeng X, Chen X, Qin H, Han Y, Chen X, Han Z, Zhao W (2020) Preventive effects of a natural anti-inflammatory agent salvianolic acid a on acute kidney injury in mice. Food Chem Toxicol 135:110901

    Article  CAS  Google Scholar 

Download references

Funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1A6A1A03033553).

Author information

Authors and Affiliations

  1. College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China

    Lei Wang & Xiaoting Fu

  2. Department of Marine Life Sciences, Jeju National University, Jeju City, Jeju Self-Governing Province, 63243, Republic of Korea

    Lei Wang, Hye-Won Yang & You-Jin Jeon

  3. Marine Science Institute, Jeju National University, Jeju City, Jeju Self-Governing Province, 63333, Republic of Korea

    Lei Wang & You-Jin Jeon

  4. Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea

    Jae-Young Oh

  5. Department of Food Science and Nutrition, Pukyong National University, 45 Yongso-ro, Nam-gu, Busan, 48513, Republic of Korea

    Jae-Il Kim

Authors
  1. Lei Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jae-Young Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hye-Won Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaoting Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jae-Il Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. You-Jin Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jae-Il Kim or You-Jin Jeon.

Ethics declarations

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, L., Oh, JY., Yang, HW. et al. Fucoidan isolated from the popular edible brown seaweed Sargassum fusiforme suppresses lipopolysaccharide-induced inflammation by blocking NF-κB signal pathway. J Appl Phycol 33, 1845–1852 (2021). https://doi.org/10.1007/s10811-021-02390-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-021-02390-7

Keywords

Search

Navigation