Abstract
Background
It has been reported that long intergenic non-protein-coding RNA 324 (LINC00324) promotes liver cancer by upregulating Fas ligand (FasL), which is a major player in intervertebral disk degeneration (IDD), indicating the involvement of LINC00324 in IDD. This study was carried out to investigate the interaction between LINC00324 and FasL in IDD.
Methods
Plasma samples were collected from both IDD (n = 60) and healthy controls (n = 60). The expression of LINC00324 and FasL in plasma was determined by RT-qPCR. The interactions between LINC00324 and FasL in nucleus pulposus (NP) cells were analyzed by overexpression experiments.
Results
LINC00324 and FasL were upregulated in IDD patients, and they were positively correlated. After treatment, the expression levels of FasL and LINC00324 were significantly decreased. In NP cells, overexpression of LINC00324 increased the expression of FasL at both mRNA and protein levels, while overexpression of FasL did not affect the expression of LINC00324.
Conclusion
LINC00324 may upregulate FasL in IDD to promote disease progression.
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Data availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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YC, YW, and RC contributed to the study design; all authors collected the data and performed the data analysis; all authors prepared the manuscript.
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Ethical approval was given by the Ethics Committee of the Affiliated Ganzhou Hospital of Nanchang University. All patients gave their written information consent.
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Chen, Y., Wu, Y., Chen, R. et al. LncRNA LINC00324 is upregulated in intervertebral disk degeneration and upregulates FasL in nucleus pulposus cells. Mol Cell Biochem 476, 1995–2000 (2021). https://doi.org/10.1007/s11010-021-04058-9
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DOI: https://doi.org/10.1007/s11010-021-04058-9