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Loss of viability and vigour in the course of short-term storage of Pityrocarpa moniliformis seeds cannot be attributed to reserve degradation

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Abstract

The quality of forest seeds may be maintained in the course of storage for their use in the restoration of degraded areas and the conservation of genetic resources. In this sense, physiological and biochemical markers were evaluated during the short-term storage of Pityrocarpa moniliformis seeds, a pioneer species native to the Caatinga. Seeds harvested from 25 mother trees were stored in a growth chamber (27 ± 4 °C) or in a refrigerator (6 ± 3 °C) for 360 days. Viability, vigour, reserve contents, metabolite contents, and hydrolase activities were assessed at the beginning of the experiment and every 60 days. Over time, a decrease in germination percentage, germination speed index, and seedling growth accompanied by an increase in membrane damage was verified in the seeds kept in a growth chamber. These alterations were not associated with the degradation of starch and storage proteins, although there was a decrease in the content of non-reducing sugars in the seeds kept in the growth chamber. Therefore, the loss of viability and vigour in P. moniliformis seeds stored for 360 days in a growth chamber cannot be attributed to the hydrolysis of major reserves, but it may be related to the accumulation of reducing sugars.

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Abbreviations

AGEs:

Advanced glycation end-products

DW:

Dry weight

FW:

Fresh weight

GSI:

Germination speed index

NRS:

Non-reducing sugars

RS:

Reducing sugars

RH:

Relative humidity

ROS:

Reactive oxygen species

TFAA:

Total free amino acids

TSS:

Total soluble sugars

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Acknowledgements

We thank to the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the schoolarships provided to F.C.F., D.F.A.O and C.S.F.

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Authors and Affiliations

  1. Unidade Acadêmica Especializada em Ciências Agrárias – UAECIA, Universidade Federal do Rio Grande do Norte, Km 03, Caixa Postal 07, Distrito de Jundiaí, Macaíba, Rio Grande do Norte, CEP 59280-000, Brazil

    Francival Cardoso Felix, Cibele dos Santos Ferrari & Mauro Vasconcelos Pacheco

  2. Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Campus Universitário, Lagoa Nova, Natal, Rio Grande do Norte, CEP 59078-970, Brazil

    Danilo Flademir Alves de Oliveira & Eduardo Luiz Voigt

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  1. Francival Cardoso Felix
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Correspondence to Eduardo Luiz Voigt.

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Felix, F.C., Alves de Oliveira, D.F., dos Santos Ferrari, C. et al. Loss of viability and vigour in the course of short-term storage of Pityrocarpa moniliformis seeds cannot be attributed to reserve degradation. New Forests 52, 237–248 (2021). https://doi.org/10.1007/s11056-020-09792-4

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