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Haemato-biochemical alterations and oxidative stress associated with naturally occurring porcine circovirus2 infection in pigs

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Abstract

Porcine circovirus2 (PCV2) infection in pigs is one of the major causes of economic loss to the farmers in terms of low production, slow growth and increase post-weaning mortality rate. The effect of PCV2 infection on haemogram, serum biochemical profile and oxidant/anti-oxidant status is not well established in pigs. In the present study, haemogram, serum biochemical profile and oxidant/anti-oxidant status were assessed in pigs confirmed positive for PCV2 infections as evidenced by commercially available enzyme-linked immunosorbent assay kit (n = 151) and polymerase chain reaction (PCR) (n = 42) among a total of 306 number of pigs included in the study. Non-infected healthy pigs (n = 6) served as healthy control. The total erythrocyte count (TEC), haemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC), differential leukocyte count (DLC) and thrombocyte count were measured. The levels of total protein, albumin, globulin, total bilirubin, direct bilirubin, blood urea nitrogen (BUN), creatinine and glucose and enzymes viz. alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyl transferase (GGT) and alkaline phosphatase (ALP) were measured. Oxidative stress indicators such as plasma malondialdehyde (MDA) and total anti-oxidant activity (TAOA) were measured using commercially available kits. The mean values of TLC, lymphocytes and thrombocyte count were significantly (P < 0.05) low in PCV2-infected pigs. The levels of globulin, AST, GGT, BUN and creatinine were significantly increased (P < 0.05) whereas levels of albumin and glucose significantly (P < 0.05) decreased in PCV2-infected pigs. The significant increase (P < 0.05) in MDA level and significant decrease (P < 0.05) in TAOA level were noticed in PCV2-infected animals as compared with healthy control. The present study supports immunosuppression, possible multiple organ damage and oxidative stress associated with naturally occurring PCV2 infection in pigs. Timely vaccination and managemental practices can reduce PCV2 infection in farms. In spite of many research studies, there is still paucity of detailed systemic study on haemato-biochemical alteration and oxidative stress associated with PCV2 infection.

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Authors and Affiliations

  1. Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, Mizoram, 796015, India

    J. B. Rajesh, H. Prasad, K. Sarma, G. E. Chethan & S. Behera

  2. Indian Council for Agricultural Research-National Research Centre on Pig, Rani, Guwahati, Assam, 781131, India

    S. Rajkhowa & N. H. Mohan

  3. Division of Medicine, Indian Council for Agricultural Research-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India

    U. Dimri

  4. All India Coordinated Research Project on Pigs, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, Mizoram, 796015, India

    L. Hmar &  Zosangpuii

  5. Department of Veterinary Biochemistry and Physiology, College of Veterinary Sciences and Animal Husbandry, Selesih, Aizawl, Mizoram, 796015, India

    P. Behera & G. Jaganmohanarao

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  1. J. B. Rajesh
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  2. S. Rajkhowa
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Rajesh, J.B., Rajkhowa, S., Dimri, U. et al. Haemato-biochemical alterations and oxidative stress associated with naturally occurring porcine circovirus2 infection in pigs. Trop Anim Health Prod 52, 2243–2250 (2020). https://doi.org/10.1007/s11250-020-02247-0

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