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miR-362-3p Targets Orosomucoid 1 to Promote Cell Proliferation, Restrain Cell Apoptosis and Thereby Mitigate Hypoxia/Reoxygenation-Induced Cardiomyocytes Injury

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Abstract

This study aimed to investigate the mechanism of how miR-362-3p/orosomucoid 1 (ORM1) involved in hypoxia/reoxygenation (H/R)-induced cardiomyocytes injury. Based on data obtained from Gene Expression Omnibus (GEO) database, we revealed that ORM1 was highly expressed and positively correlated with the expression of inflammatory factors (MAPK1, MAPK3, IL1B and CASP9). miR-362-3p was identified as an upstream regulatory miRNA of ORM1 and negatively modulated the mRNA and protein expression levels of ORM1 in H/R-injured cardiomyocytes. Moreover, we found that miR-362-3p was downregulated in cardiomyocytes injured by H/R. The promoting influence of miR-362-3p mimic on the proliferation and the inhibitory effect of miR-362-3p mimic on the apoptosis of H/R-stimulated cardiomyocytes were eliminated by overexpression of ORM1. Furthermore, miR-362-3p affected the expression of MAPK1, MAPK3, IL1B and CASP9 in H/R-injured cardiomyocytes through targeting ORM1. Our outcomes illustrated that miR-362-3p exhibited a protective influence on H/R-induced cardiomyocytes through targeting ORM1.

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Data Availability

The data and material are available from the corresponding author on reasonable request.

Abbreviations

ORM1:

Orosomucoid 1

miR-362-3p:

MicroRNA-362-3p

AMI:

Acute myocardial infarction

H/R:

Hypoxia/reoxygenation

WHO:

World Health Organization

CVD:

Cardiovascular disease

MiRNAs:

MicroRNAs

MAPK1/3:

Mitogen-activated protein kinase 1/3

ERKs:

MAP kinase

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Authors and Affiliations

  1. School of Graduate, Hebei Medical University, Shijiazhuang, Hebei, 050017, P.R. China

    Meijing Shi & Xuelian Song

  2. College of Integrated Chinese and Western Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, 050200, P.R. China

    Xiuru Ma

  3. Department of Cardiology Center, Hebei General Hospital, No.348 Heping West Road, Shijiazhuang, Hebei, 050051, P.R. China

    Qian Yang, Wenjing Wang, Xinning Li, Xuelian Song, Yingxiao Li, Yuetao Xie & Yi Dang

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  1. Meijing Shi
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Shi, M., Ma, X., Yang, Q. et al. miR-362-3p Targets Orosomucoid 1 to Promote Cell Proliferation, Restrain Cell Apoptosis and Thereby Mitigate Hypoxia/Reoxygenation-Induced Cardiomyocytes Injury. Cardiovasc Toxicol 21, 387–398 (2021). https://doi.org/10.1007/s12012-020-09631-0

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