Abstract
Corncob waste management has become a concern for corn producers worldwide. Agricultural waste-based energy generation is, therefore, extensively researched these days. However, the calorific potential of the moist corncobs still remains untapped due to the lack of technological advancements. In this study, a dilute acid pre-treatment followed with enzymatic hydrolysis was used to recover 80% of the fermentable sugars from the corncobs. When used for microbial oil production in a two-stage fermentation, this enzymatic hydrolysate resulted in a lipid production of more than 75% dried cell weight (DCW) in Rhodotorula glutinis. Extracellular oil capture in this two-stage fermentation further increased the total oil production to 8 g L−1 and 80% DCW. An oleic acid-rich microbial oil profile obtained from this process exhibited excellent biofuel properties. The energy content of the microbial oil valued 40 MJ kg−1, and was found to be 2.35 times higher than the intrinsic heating capacity of corncobs. Thus, a sustainable bioprocess for corncob waste remediation was devised to obtain vegetable oil equivalent through a microbial conversion platform.
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Data availability
The data used to support the findings of this study are available from the corresponding author upon request.
Abbreviations
- ASTM:
-
American Standard for Testing Material
- CFPP:
-
cold filter plugging point
- CN:
-
cetane number
- C/N:
-
ratio of carbon to nitrogen in the media
- CV:
-
calorific value
- DCW:
-
dry cell weight
- FAME:
-
fatty acid methyl esters
- HHV:
-
high heating value
- IV:
-
iodine value
- KV:
-
kinematic viscosity
- LCSF:
-
long-chain saturation factor
- LLE:
-
liquid-liquid extraction
- MUFA:
-
monounsaturated fatty acid
- OCA:
-
oil capturing agent
- PUFA:
-
polyunsaturated fatty acid
- RPR:
-
residue production ratio
- SV:
-
saponification value
- TAG:
-
triacylglycerol
- vvm:
-
volume per volume per minute
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Acknowledgements
The authors acknowledge the Institute of Chemical Technology, Mumbai (formerly U.D.C.T., Mumbai), and DBT-ICT Centre for Energy Biosciences for providing infrastructure for carrying this research work.
Funding
DVR and PPP gratefully acknowledge the Department of Biotechnology, Government of India, and DBT-ICT Centre for Energy Biosciences, Mumbai, for their fellowships. The authors are thankful to the Department of Biotechnology, Government of India, for financial support (Project grant No.: BT/EB/ICT-Extension/2012, 05/06/2013).
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Rane, D.V., Pawar, P.P., Odaneth, A.A. et al. Microbial oil production by the oleaginous red yeast, Rhodotorula glutinis NCIM 3168, using corncob hydrolysate. Biomass Conv. Bioref. 13, 1987–1997 (2023). https://doi.org/10.1007/s13399-021-01298-z
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DOI: https://doi.org/10.1007/s13399-021-01298-z