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In vitro inhibitory effect of two commercial probiotics on chromogenic actinomycetes

  • Original Scientific Article
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European Archives of Paediatric Dentistry Aims and scope Submit manuscript

Abstract

Purpose

Black extrinsic discoloration is a common clinical and aesthetic problem. This study aims to evaluate the potential in vitro antagonistic activity of two commercial probiotics, Streptococcus salivarius M18 and Lactobacillus reuteri ProDentis, against microorganisms associated with black stains.

Methods

Streptococcus salivarius M18 and Lactobacillus reuteri were tested against Aggregatibacter actinomycetemcomitans and Actinomyces naeslundiiusing their cell-free fermentative broth in a planktonic growth inhibition test.

Results

Both probiotic cell-free supernatants showed the ability to reduce the pathogenic bacteria growth in a dose-dependent way. Streptococcus salivarius M18 showed a stronger antimicrobial activity than Lactobacillus reuteri ProDentis against the two indicator strains used. A. naeslundi was less susceptible to the probiotic activity of both S. salivarius and L. reuteri compared to A. actinomycetemcomitans.

Conclusions

The obtained results demonstrate a potent antagonistic ability of probiotics to reduce the growth of microorganisms associated with black tooth stains. Therefore, these strains could be evaluated for a therapeutic use against dental pigmentations.

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Acknowledgements

The Authors are grateful to Dr Veronica Cappa for the statistical support.

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Authors and Affiliations

  1. Agrifood and Environmental Microbiology Platform (PiMiAA-AgroFood Lab), Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy

    E. Gobbi

  2. Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy

    M. A. De Francesco, G. Piccinelli & A. Caruso

  3. Department of Medical and Surgical Specialities, Radiological Sciences and Public Health, Dental School, Dental Clinic, University of Brescia, p.le Spedali Civili 1, 25 133, Brescia, Italy

    E. Bardellini & A. Majorana

Authors
  1. E. Gobbi
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  2. M. A. De Francesco
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  3. G. Piccinelli
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  4. A. Caruso
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  5. E. Bardellini
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  6. A. Majorana
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Correspondence to E. Bardellini.

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The Authors declare that they have no conflict of interest.

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This study did not involve any human participants or animals.

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This study did not need informed consent from the patients, being an in vitro study.

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Gobbi, E., De Francesco, M.A., Piccinelli, G. et al. In vitro inhibitory effect of two commercial probiotics on chromogenic actinomycetes. Eur Arch Paediatr Dent 21, 673–677 (2020). https://doi.org/10.1007/s40368-020-00512-2

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  • DOI: https://doi.org/10.1007/s40368-020-00512-2

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