Skip to main content
SpringerLink
Log in

The Improved Expression and Stability of Zearalenone Lactonohydrolase from Escherichia coli BL21 (DE3)

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

Zearalenone (ZEN) is an estrogenic mycotoxin produced by Fusarium fungi which is commonly found in cereal crops. Because of its estrogen toxicity, ZEN can cause reproductive disorders and reduce immune function in human and animals. The zearalenone lactonohydrolase (ZHD 101) derived from Clonostachys rosea IFO 7063 can efficiently hydrolyze and detoxify ZEN. However, application of ZHD101 was always limited because of its instability and poor solubility. In this study, a codon-optimized DNA fragment for a recombinant dissolved ZHD101 (rdZHD) was designed and synthesized, and soluble rdZHD protein was successfully expressed and purified using Escherichia coli BL21 (DE3) expression system. HPLC results showed that 100 μg/mL ZEN could be thoroughly degraded by rdZHD within 6 h, and the ZEN degradation activity of rdZHD was calculated as 1150 U/mg. Furthermore, the purified rdZHD was immobilized with cross-linked poly(γ-glutamic acid)/gelatin hydrogel (CPE), and the activity and stability of immobilized rdZHD (CPE-rdZHD) was evaluated. The CPE-rdZHD showed better pH stability and thermostability than free rdZHD. The research could provide an effectively biological detoxification technology for ZEN degradation in agriculture and grain processing industry.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Zinedine, A., Soriano, J.M., Moltó, J.C., and Mañes, J., Food Chem. Toxicol., 2007, vol. 45, no. 1, pp. 1–18.

    Article  CAS  Google Scholar 

  2. Kakeya, H., Takahashi-Ando, N., Kimura M., Onose, R., Yamaguchi, I., and Osada, H., Biosci. Biotechnol. Biochem., 2002, vol. 66, no. 12, pp. 2723–2726.

    Article  CAS  Google Scholar 

  3. Minervini, F. and Dell’Aquila M.E., Int. J. Mol. Sci., 2008, vol. 9, no. 12, pp. 2570–2584.

    Article  CAS  Google Scholar 

  4. Gallo, A., Giuberti G., Frisvad, J.C., Bertuzzi, T., and Nielsen, K.F., Toxins (Basel), 2015, vol.7, no. 8, pp. 3057–3111.

    Article  CAS  Google Scholar 

  5. Chilaka, C.A., De Boevre, M., Atanda, O.O., and De Saeger, S., Toxins (Basel), 2017, vol. 9, no. 1, p. 19.

    Article  Google Scholar 

  6. Hassan, Y.I. and Zhou, T., Toxins (Basel), 2018, vol. 10, no. 3, p. 116.

    Article  Google Scholar 

  7. Shanakhat, H., Sorrentino, A., Raiola, A., Romano, A., Masi, P., and Cavella, S., J. Sci. Food Agric., 2018, vol. 98, no. 11, pp. 4003–4013.

    Article  CAS  Google Scholar 

  8. Awad, W.A., Ghareeb, K., Bohm, J., and Zentek, J., Food Addit. Contam. A, 2010, vol. 27, no. 4, pp. 510–520.

    Article  CAS  Google Scholar 

  9. Li, S.Y., Yu, Q.X., Xiang, L., Zhou, Y.L., and Zhang, G.M., Chin. J. Biotech., 2018, vol. 34, no. 4, pp. 489–500.

    Google Scholar 

  10. Zhu, Y., Hassan, Y.I., Lep,p D., Shao, S., and Zhou, T., Toxins (Basel), 2017, vol. 9, no. 4, p. 130.

    Article  Google Scholar 

  11. Takahashi-Ando, N., Kimura, M., Kakeya, H., Osada, H., and Yamaguchi, I., Biochem. J., 2002, vol. 365, no. 1, pp. 1–6.

    Article  CAS  Google Scholar 

  12. Xiang, L., Wang, Q.H., Zhou, Y.L., Yin, L.F., Zhang, G.M., and Ma, Y.H., Microbiol. Res., 2016, vol. 193, pp. 48–56.

    Article  CAS  Google Scholar 

  13. Sirisha, V.L., Jain, A., and Jain, A., Adv. Food Nutr. Res., 2016, vol. 79, pp. 179–211.

    Article  CAS  Google Scholar 

  14. He, M.L., Li, Y., Pi, F.W., Ji, J., He, X.X., Zhang, Y.Z., and Sun, X.L., Food Control, 2016, vol. 68, pp. 271–279.

    Article  CAS  Google Scholar 

  15. Chang, S.W., Shaw, J.F., Yang, K.H., Chang, S.F., and Shieh, C.J., Biores. Technol., 2008, vol. 99, no. 8, pp. 2800–2805.

    Article  CAS  Google Scholar 

  16. Xie, J., Zhang, H., Li, X., and Shi, Y., Biomacromolecules, 2014, vol. 15, no. 2, pp. 690–697.

    Article  CAS  Google Scholar 

  17. Yang, L.Q., Li, X.Y., Li, X., Su, Z.C., Zhang, C.G., Xu, M.K., and Zhan,g H.W., Biomacromolecules, 2015, vol. 287, pp. 287–295.

    CAS  Google Scholar 

  18. Lai, E.P., Wang, Y.X., Wei, Y., Li, G., and Ma, G.H., J. Appl. Polym. Sci., 2016, vol. 133, no. 21, pp. 1–9.

    Article  Google Scholar 

  19. Baneyx, F. and Mujacic, M., Nat. Biotechnol., 2004, vol. 22, no. 11, pp. 1399–1408.

    Article  CAS  Google Scholar 

  20. Phadtare, S. and Severinov, K., J. Bacteriol., 2005, vol. 187, no. 18, pp. 6584–6589.

    Article  CAS  Google Scholar 

  21. Gomez, C.G., Alvarez Igarzabal, C.I., and Strumia, M.C., Polymer, 2004,vol. 45, no. 18, pp. 6189–6194.

    Article  CAS  Google Scholar 

  22. Sheldon, R.A., Adv. Synth. Catal., 2007, vol. 349, no. 8–9, pp. 1289–1307.

    Article  CAS  Google Scholar 

  23. Keerti Gupta, A., Kumar, V., Dubey, A., and Verma, A.K., I.S.R.N. Biochem., 2014, vol. 2014, p. 178498.

    Google Scholar 

  24. Sahin, F., Demirel, G., and Tümtürk, H., Int. J. Biol. Macromol., 2005, vol. 37, no. 3, pp. 148–153.

    Article  CAS  Google Scholar 

  25. Bezbradica, D.I., Mateo, C., and Guisan, J.M., J. Mol. Catal. B: Enzym., 2014, vol.102, pp. 218–224.

    Article  CAS  Google Scholar 

  26. Wang, N., Wu, R.N., Jiang, S.W., Hart, D.J., Gao, H.R., Li, X.T., Lian, X.L., and An, Y.F., Chem. Eng. Technol., 2018, vol. 41, pp. 1949–1955.

    Article  CAS  Google Scholar 

  27. Huang, X.J., Ge, D., and Xu, Z.K., Eur. Polym., 2007, vol. 43, no. 9, pp. 3710–3718.

    Article  CAS  Google Scholar 

Download references

Funding

This research was supported by National Key Research and Development Program of China (2017YFD0800705), National Natural Science Foundation of China (41877503), Liaoning Revitalization Talents Program (XLYC1807226), and Shenyang High-level Innovative Talents Program (RC190060).

Author information

Authors and Affiliations

  1. Institute of Applied Ecology, Chinese Academy of Sciences, 110016, Shenyang, PR China

    X. Fu, M. Xu, Y. Li, H. Zhang & C. Zhang

  2. Shenyang Research Institute of Chemical Industry, 110021, Shenyang, PR China

    T. Li

Authors
  1. X. Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to X. Fu or M. Xu.

Ethics declarations

The authors declare no financial or commercial conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fu, X., Xu, M., Li, T. et al. The Improved Expression and Stability of Zearalenone Lactonohydrolase from Escherichia coli BL21 (DE3). Appl Biochem Microbiol 57, 79–85 (2021). https://doi.org/10.1134/S0003683821010075

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683821010075

Keywords:

Search

Navigation