Abstract
In experiments on SPF mice, the authors evaluated the radio-protective effectiveness of the recombinant Flagellin, developed at the Institute of High-Purity Preparations (St. Petersburg), administered at a wide range of doses of 0.004–2 mg/kg for 30 min prior to X-ray irradiation in the dose range of 7–10 Gy. The effectiveness of Flagellin was evaluated in terms of 30-day survival rates of the mice, the average life expectancy, and according to the DMF criterion for bone marrow death. The DMF values were 1.24–1.23–1.22 at the level of LD16, LD50, and LD84. ED50 approximately equals 25 μg/kg. The dose-response effect of Flagellin was evaluated to reach a plateau at a dose of 0.2 mg/kg.
Similar content being viewed by others
REFERENCES
Burdelya, L.G., Krivokrysenko, V.I., Tallant, T.C., et al., An agonist of toll-lair receptor 5 has radioprotective activity in mouse and primate models, Science, 2008, vol. 320, no. 5873, pp. 226–230.
Krivokrysenko, V.N., Shakhov, A.N., Gudkov, A.V., and Feinstein, E., Identification of G-CSF and IL-6 as candidate biomarkers of GBLB502 efficacy as a medical radiation countermeasure, JPET Fast Forward, 2012, pp. 1–46. https://doi.org/10.1124/jpet.112.196071
Al’-Shekhadat, R.I., Matyunina, E.A., Sharafutdinova, T.A., et al., Obtaining and studying the properties of recombinant bacterial flagellin, Khim. Radiats. Bezop., 2012, spec. issue, pp. 109–116.
Grebenyuk, A.N., Aksenova, N.V., Petrov, A.V., et al., Obtaining various variants of recombinant flagellin and assessing their radioprotective effectiveness, Vestn. Ros. Voen.-Med. Akad., 2013, vol. 43, no. 3, pp. 75–80.
Sofronov, G.A., Murzina, E.V., Bolekhan, V.N., et al., Promising directions for the use of recombinant flagellin-based drugs, Med. Akad. Zh., 2017, no. 2, pp. 7–20.
Murzina, E.V., Sofronov, G.A., Aksenova, N.V., et al., Antiradiation properties of bacterial flagellin, Izv. Ros. Voen.-Med. Akad., 2017, vol. 36, no. 2, suppl. 1, р. 242.
Rozhdestvenskii, L.M., Konchalovskii, M.V., Baranov, A.E., et al., Possibility of using the drug Betaleukin (interleukin-1β) for emergency therapeutic care in acute emergency human exposure, in V Ross. Nats. Kongr. “Chelovek i lekarstvo”, Moskva, 21–25 aprelya 1998 (V Russ. Natl. Congr. “Man and Medicine,” Moscow, April 21–25, 1998), Moscow, 1998, p. 454.
Direktiva 2010/63/EU Evropeiskogo parlamenta i Soveta evropeiskogo soyuza po okhrane zhivotnykh, ispol’zuemykh v nauchnykh tselyakh (Directive 2010/63/ EU of the European Parliament and the Council of the European Union on the Protection of Animals Used for Scientific Purposes), St. Petersburg: Rus-Lasa NP Ob’’edinenie Spetsialistov po Rabote s Laboratornymi Zhivotnymi, 2012.
Popova, E.B., Research planning and analysis of dose–effect relationships for toxic and medicinal compounds, Doctoral (Med.) Dissertation, St. Petersburg, 2010.
O’Neill, L. and Green, C., Signal transduction pathways activated by the IL-1 receptor family: ancient signaling machinery in mammals, insects, and plants, J. Leukocyte Biol., 1998, vol. 63, pp. 650–657.
Neta, R., Cytokines in radioprotection and therapy of radiation injury, Biotherapy, 1988, no. 1, pp. 41–45.
Grebenyuk, A.N. and Legeza, V.I., Protivoluchevye svoistva interleikina-1 (Anti-Radiation Properties of Interleukin-1), St. Petersburg: OOO Izd. Foliant, 2012.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest. The authors declare that they have no conflict of interest.
Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Rights and permissions
About this article
Cite this article
Lisina, N.I., Shchegoleva, R.A., Shlyakova, T.G. et al. Evaluation of the Antiradiation Efficiency of Flagellin in Experiments on Mice. Biol Bull Russ Acad Sci 47, 1531–1535 (2020). https://doi.org/10.1134/S1062359020110084
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1062359020110084