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Preparation of Tissue-Specific Matrix from Decellularized Porcine Cartilage

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Abstract

For full decellularization of cartilage tissue when preparing tissue-specific matrices of dense tissues from decellularized porcine articular cartilage, treatment with surfactant should be followed by treatment with supercritical fluids. Ethanol introduced as a polarity modifier leads to more complete cartilage decellularization, which is associated with a reduction of destructive changes in the extracellular matrix. The preserved morphology and active proliferation of mesenchymal stromal cells of human adipose tissue in the presence of the tissue-specific matrix of porcine cartilage indicate that the matrix is promising for use as a cell carrier for tissue engineering of cartilage.

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Funding

This study was partially supported by the Russian Foundation for Basic Research (grant no. 18-29-06012).

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Authors and Affiliations

  1. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182, Moscow, Russia

    E. A. Nemets, Yu. B. Basok, L. A. Kirsanova, A. D. Kirillova & V. I. Sevastianov

  2. Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    A. E. Lazhko

  3. Faculty of Chemistry, Moscow State University, 119991, Moscow, Russia

    A. E. Lazhko

Authors
  1. E. A. Nemets
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  2. A. E. Lazhko
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  3. Yu. B. Basok
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  4. L. A. Kirsanova
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  5. A. D. Kirillova
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  6. V. I. Sevastianov
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Corresponding author

Correspondence to E. A. Nemets.

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Translated by L. Smolina

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Nemets, E.A., Lazhko, A.E., Basok, Y.B. et al. Preparation of Tissue-Specific Matrix from Decellularized Porcine Cartilage. Russ. J. Phys. Chem. B 14, 1245–1251 (2020). https://doi.org/10.1134/S1990793120080059

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  • DOI: https://doi.org/10.1134/S1990793120080059

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