Skip to main content
SpringerLink
Log in

Significant Effects in Bread-making Quality Associated with the Gene Cluster Glu-D3/Gli-D1 from the Bread Wheat Cultivar Prointa Guazú

  • Quality and Utilization
  • Published:
Cereal Research Communications Aims and scope Submit manuscript

Abstract

Seed storage proteins (gliadins and glutenins) play a key role in the determination of dough and bread-making quality in bread wheat. This is due to the interaction between high and low molecular weight glutenins subunits and gliadins, via complex inter- and intramolecular bondings. In contrast to high molecular weight glutenins, low molecular weight glutenins and gliadins analysis is difficult due to the large number of expressed subunits and coding genes. For these reasons the role of individual proteins/subunits in the determination of wheat quality is less clear. In this work we studied the effect of gene clusters Glu-A3/ Gli-A1 and Glu-D3/Gli-D1 in bread-making quality parameters using 20 F4-6 families from the cross Prointa Guazú × Prointa Oasis, both cultivars carrying identical high molecular weight glutenins subunits composition and presence of 1BL/1RS wheat-rye translocation, but differing in Glu-A3/Glu-D3 low molecular weight glutenins subunits and Gli-A1/Gli-D1 gliadins patterns. ANCOVA analysis showed a significant contribution of the Glu-D3/Gli-D1 gene cluster provided by Prointa Guazú to gluten strength explained by mixograph parameters MDS and PW, and Zeleny Test. Markers tagging Prointa Guazú Glu-D3/Gli-D1 alleles are available for strong gluten selection in breeding programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • AACC. 2000. American Association of Cereal Chemists approved methods of analysis 10th ed. St. Paul, MN, USA.

    Google Scholar 

  • Bonafede, M.D., Tranquilli, G., Pflüger, L.A., Peña, R.J., Dubcovsky, J. 2015. Effect of allelic variation at the Glu-3/Gli-1 loci on breadmaking quality parameters in hexaploid wheat (Triticum aestivum L.). J. Cereal Sci. 62:143–150.

    Article  CAS  Google Scholar 

  • Branlard, G., Dardevet, M. 1985. Diversity of grain proteins and bread wheat quality: I. Correlation between gliadin bands and flour quality characteristics. J. Cereal Sci. 3:329–343.

    Article  CAS  Google Scholar 

  • Branlard, G., Dardevet, M., Saccomano, R. 2001. Genetic diversity of wheat storage proteins and bread wheat quality. Euphytica 119:59–67.

    Article  CAS  Google Scholar 

  • Brody, J.R., Kern, S.E. 2004. Sodium boric acid: A Tris-free, cooler conductive medium for DNA electrophoresis. Biotechniques 36:214–216.

    Article  CAS  Google Scholar 

  • Butow, B.J., Gale, K.R., Ikea, J., Juhász, A., Bedő, Z., Tamás, L., Gianibelli, M.C. 2004. Dissemination of the highly expressed Bx7 glutenin subunit (Glu-B1al allele) in wheat as revealed by novel PCR markers and RP-HPLC. Theor. Appl. Genet. 109:1525–1535.

    Article  CAS  Google Scholar 

  • Caballero, L., Martín, L.M., Alvarez, J.B. 2004. Genetic variability of the low-molecular-weight glutenin subunits in spelt wheat (Triticum aestivum ssp. spelta L. em Thell.). Theor. Appl. Genet. 108:914–919.

    Article  CAS  Google Scholar 

  • D’Ovidio, R., Masci, S. 2004. The low-molecular-weight glutenin subunits of wheat gluten. J. Cereal Sci. 39:321–339.

    Article  Google Scholar 

  • Espí, A., Rodríguez-Quijano, M., Vázquez, J.F., Carrillo, J.M., Giraldo, P. 2014. Molecular characterization of Glu-B3 locus in wheat cultivars and segregating populations. J. Cereal Sci. 60:374–381.

    Article  Google Scholar 

  • Gao, X., Appelbee, M.J. Mekuria, G.T., Chalmers, K.J., Mather, D.E. 2012. A second “overexpression” allele at the Glu-B1 high-molecular-weight glutenin locus of wheat: Sequence characterisation and functional effects. Theor. Appl. Genet. 124:333–343.

    Article  CAS  Google Scholar 

  • Gianibelli, M.C., Echaide, M., Larroque, O.R., Carrillo, J.M., Dubcovsky, J. 2002. Biochemical and molecular characterisation of Glu-1 loci in Argentinean wheat cultivars. Euphytica 128:61–73.

    Article  CAS  Google Scholar 

  • Graybosch, R.A. 2001. Uneasy unions: Quality effects of rye chromatin transfers to wheat. J. Cereal Sci. 33:3–16.

    Article  CAS  Google Scholar 

  • Graybosch, R.A., Peterson, C.J., Hansen, L.E., Worrall, D., Shelton, D.R., Lukaszewski, A. 1993. Comparative Flour Quality and Protein Characteristics of 1BL/1RS and 1AL/1RS wheat-rye translocation lines. J. Cereal Sci. 17:95–106.

    Article  CAS  Google Scholar 

  • Hrušková, M., Švec, I., Jirsa, O. 2006. Correlation between milling and baking parameters of wheat varieties. J. Food Eng. 77:439–444.

    Article  Google Scholar 

  • Ibba, M.I., Kiszonas, A.M., Guzmán, C., Morris, C.F. 2017. Definition of the low molecular weight glutenin subunit gene family members in a set of standard bread wheat (Triticum aestivum L.) varieties. J. Cereal Sci. 74:263–271.

    Article  CAS  Google Scholar 

  • Khan, K., Hamadar, A., Patek, J. 1985. Polyacrilamide gel electrophoresis for wheat variety identification: effect of variables on gel properties. Cereal Chem. 62:310–313.

    Google Scholar 

  • Lerner, S.E., Kolman, M.A., Rogers, W.J. 2009. Quality and endosperm storage protein variation in Argentinean grown bread wheat. I. Allelic diversity and discrimination between cultivars. J. Cereal Sci. 49:337–345.

    Article  CAS  Google Scholar 

  • Lookhart, G.L., Hagman, K., Kasarda, D.D. 1993. High molecular weight glutenin subunits of the most commonly grown wheat cultivars in the U.S. in 1984. Plant Breed. 110:48–62.

    Article  CAS  Google Scholar 

  • Lukow, O.M., Payne, P.I., Tkachuk, R. 1989. The HMW glutenin subunit composition of Canadian wheat cultivars and their association with bread making quality. J. Sci. Food Agric. 46:451–460.

    Article  CAS  Google Scholar 

  • Martinant, J.P., Nicolas, Y., Bouguennec, A., Popineau, Y., Soulnier, L., Branlard, G. 1998. Relationships between Mixograph parameters and indices of wheat grain quality. J. Cereal Sci. 27:179–189.

    Article  CAS  Google Scholar 

  • Masci, S.M., Porceddu, E., Colaprico, G., Lafiandra, D. 1991. Comparison of the B and D subunits of glutenin encoded at the Glu-D3 locus in two biotypes of the common wheat cultivar Newton with different technological characteristics. J. Cereal Sci. 14:35–46.

    Article  CAS  Google Scholar 

  • Moiraghi, M., Vanzetti, L., Pflüger, L., Helguera, M., Pérez, G.T. 2013. Effect of high molecular weight glutenins and rye translocations on soft wheat flour cookie quality. J. Cereal Sci. 58:424–430.

    Article  CAS  Google Scholar 

  • Nakamura, H., Inazu, A., Hirano, H. 1999. Allelic variation in high-molecular-weight glutenin subunit Loci of Glu-1 in Japanese common wheats. Euphytica 106:131–138.

    Article  CAS  Google Scholar 

  • Payne, P.I. 1987. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu. Rev. Plant Physiol. 38:141–153.

    Article  CAS  Google Scholar 

  • Payne, P.I., Nightingale, M.A., Krattiger, A.F., Holt, L.M. 1987. The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. J. Sci. Food Agric. 40:51–65.

    Article  CAS  Google Scholar 

  • Pflüger, L.A., D’Ovidio, R., Margiotta, B., Peña, R., Mujeeb-Kazi, A., Lafiandra, D. 2001. Characterisation of high- and low-molecular weight glutenin subunits associated to the D genome of Aegilops tauschii in a collection of synthetic hexaploid wheats. Theor. Appl. Genet. 103:1293–1301.

    Article  Google Scholar 

  • Di Rienzo, J.A., Casanoves, F., Balzarini, M.G., Gonzalez, L., Tablada, M., Robledo, C.W. 2016. InfoStat versión 2016. FCA UNC, Argentina, http://www.infostat.com.ar.

    Google Scholar 

  • Rogers, W., Payne, P., Harinder, K. 1989. The HMW glutenin subunit and gliadin compositions of German grown varieties and their relationship with bread-making quality. Plant Breed. 103:89–100.

    Article  CAS  Google Scholar 

  • Singh, N.K., Shepherd, K.W. 1988. Linkage mapping of genes controlling endosperm storage proteins in wheat. Theor. Appl. Genet. 75:628–641.

    Article  CAS  Google Scholar 

  • Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B.C., Remm, M., Rozen, S.G. 2012. Primer3-new capabilities and interfaces. Nucleic Acids Res. 40:1–12.

    Article  Google Scholar 

  • Wining, S., Langridge, P. 1991. Identification and mapping of polymorphism in cereals based on polymerase chain reaction. Theor. Appl. Genet. 82:209–216.

    Article  Google Scholar 

  • Woychik, J.H., Boundy, J.A., Dimler, R.J. 1961. Starch gel electrophoresis of wheat gluten proteins with concentrated urea. Arch. Biochem. Biophys. 94:477–482.

    Article  CAS  Google Scholar 

  • Zhang, W., Gianibelli, M.C., Rampling, L.R., Gale, K.R. 2004. Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum L). Theor. Appl. Genet. 108:1409–1419.

    Article  CAS  Google Scholar 

  • Zhang, X., Liu, D., Jiang, W., Guo, X., Yang, W., Sun, J., Ling, H., Zhang, A. 2011a. PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 123:1293–1305.

    Article  CAS  Google Scholar 

  • Zhang, X., Liu, D., Yang, W., Liu, K., Sun, J., Guo, X., Li, Y., Wang, D., Ling, H., Zhang, A. 2011b. Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.). Theor. Appl. Genet. 122:1503–1516.

    Article  CAS  Google Scholar 

  • Zhang, X., Liu, D., Zhang, J., Jiang, W., Luo, G., Yang, W., Sun, J., Tong, Y., Cui, D., Zhang, A. 2013. Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat. J. Exp. Bot. 64:2027–2040.

    Article  CAS  Google Scholar 

  • Zhao, H., Wang, R., Guo, A., Hu, S., Sun, G. 2004. Development of primers specific for LMW-GS genes located on chromosome 1D and molecular characterization of a gene from Glu-D3 complex locus in bread wheat. Hereditas 141:193–198.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. INTA EEA Marcos Juárez, Ruta 12 S/N, 2580, Marcos Juárez, Córdoba, Argentina

    M. Demichelis, L. S. Vanzetti, J. M. Crescente, M. M. Nisi, C. T. Bainotti, M. B. Cuniberti, L. R. Mir & M. Helguera

  2. CONICET, Av. Rivadavia 1917 C1033AAJ CABA, Argentina

    L. S. Vanzetti & J. M. Crescente

  3. Instituto de Recursos Biológicos INTA Castelar, Las Cabañas y Los Reseros S/N, 1686, Hurlingham, Buenos Aires, Argentina

    L. Pflüger

Authors
  1. M. Demichelis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. S. Vanzetti
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. M. Crescente
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. M. Nisi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Pflüger
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. T. Bainotti
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. B. Cuniberti
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. L. R. Mir
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. M. Helguera
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Helguera.

Additional information

Communicated by F. Békés

Electronic supplementary material

42976_2019_4701111_MOESM1_ESM.pdf

Significant Effects in Bread Making Quality Associated with the Gene Cluster Glu-D3/Gli-D1 from the Bread Wheat Cultivar Prointa Guazú

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Demichelis, M., Vanzetti, L.S., Crescente, J.M. et al. Significant Effects in Bread-making Quality Associated with the Gene Cluster Glu-D3/Gli-D1 from the Bread Wheat Cultivar Prointa Guazú. CEREAL RESEARCH COMMUNICATIONS 47, 111–122 (2019). https://doi.org/10.1556/0806.46.2018.055

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1556/0806.46.2018.055

Keywords

Search

Navigation