Abstract
Gnathopogon herzensteini is a small-sized fish endemic to the upper reaches of the Jialing River and the Han River. Wild populations of G. herzensteini have declined sharply during the past few decades and the phylogeny of this species has not yet fully resolved. Notably, the relationship between Gnathopogon and Gobiocypris genera still remains controversial. Scientific progress is hampered by the scarcity of molecular data for G. herzensteini. To address this problem, the complete mitochondrial genome of G. herzensteini were sequenced and characterised, and used to analyse its phylogenetic position within the subfamily Gobioninae. The genome is a circular molecule of 16,597 bases, consisting of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region (D-loop). The nucleotide composition exhibited A/T bias: A 30.0%, T 27.0%, G 17.2%, and C 25.8%. Phylogenetic tree revealed that Gobiocypris clustered within the genus Gnathopogon, thus rendering it paraphyletic. The clade of genus Gnathopogon was closely related to Coreoleuciscus, with strong statistical support. These data are a stepping stone towards the understanding of genetics and phylogeny of G. herzensteini.
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Abbreviations
- AIC:
-
Akaike information criterion
- ATP6:
-
ATP synthase F0 subunit 6
- ATP8:
-
ATP synthase F0 subunit 8
- bp:
-
base pair(s)
- BI:
-
Bayesian Inference
- cyt b:
-
cytochrome b
- COX1:
-
cytochrome c oxidase subunit 1
- COX2:
-
cytochrome c oxidase subunit 2
- COX3:
-
cytochrome c oxidase subunit 3
- H-strand:
-
heavy strand
- ML:
-
Maximum Likelihood
- MP:
-
Maximum Parsimony
- ND1:
-
NADH Dehydrogenase Subunit 1
- ND2:
-
NADH Dehydrogenase Subunit 2
- ND3:
-
NADH Dehydrogenase Subunit 3
- ND4:
-
NADH Dehydrogenase Subunit 4
- ND4L:
-
NADH dehydrogenase subunit 4L
- ND5:
-
NADH Dehydrogenase Subunit 5
- ND6:
-
NADH Dehydrogenase Subunit 6
- NUC:
-
13 PCGs and 2 rRNA genes (12 s RNA and 16 s RNA)
- PCG:
-
protein-coding gene
- rRNA:
-
ribosomal RNA
- RAG1:
-
exon 3 of recombination activating gene 1
- tRNA:
-
transfer RNA
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Acknowledgements
We thank Zhenyu Lv for his help in sample collection and identification.
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This research was supported by the National Natural Science Foundation of China (No. 51,779,210), and the Fundamental Research Funds of China West Normal University (No. 19C007, No. CXTD2016-3). The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.
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Mao, L., Zeng, Y., Li, J. et al. The complete mitochondrial genome sequence and phylogenetic analysis of Gnathopogon herzensteini(Cypriniformes, Cyprinidae, Gobioninae). Biologia 76, 1087–1094 (2021). https://doi.org/10.2478/s11756-020-00667-9
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DOI: https://doi.org/10.2478/s11756-020-00667-9